Protein Carbonyl ELISA Kit

Properties

use: sufficient for 100 colorimetric tests

Detection method: colorimetric

Storage temperature: 2-8 ° C

Related categories

Metabolism test kits

General description

Oxidative stress occurs when the effectiveness of antioxidant defenses is insufficient to cope with the production of reactive oxygen species (ROS). ROS can cause damage to DNA, lipids, and proteins. Protein oxidation results in the production of stable carbonyl groups, which can be used as a measure of oxidative damage.

Request

A protein carbonyl content assay kit has been used to determine the protein carbonyl concentration.

Suitability

Suitable for quantifying carbonyls in protein samples.

Beginning

The Protein Carbonyl Content Assay Kit provides a simple and straightforward procedure for measuring carbonyl content in a variety of biological samples. The carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) which leads to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

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