OxiSelect TBARS Assay Kit (MDA
OxiSelect TBARS, Assay Kit
Full Product Name
OxiSelect TBARS Assay Kit (MDA Quantification)
Product synonym names
OxiSelect TBARS Assay Kit (MDA Quantification)
Preparation and storage
Store all components at 4 degrees C until their expiration date.
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved. If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Occasionally, small volumes of OxiSelect TBARS Assay Kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Related Product Information for OxiSelect TBARS Test Kit
Assay Principle: The Thiobarbituric Acid Reactive Substances Assay Kit (TBARS) is a tool for the direct quantitative measurement of MDA in biological samples. Unknown samples containing MDA or MDA standards are first reacted with TBA at 95 degrees C. After a short incubation, samples and standards can be read spectrophotometrically or fluorometrically. The MDA content in unknown samples is determined by comparison with the predetermined MDA standard curve.
Background: lipid peroxidation is a well-defined mechanism of cell damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that break down to form more complex and reactive compounds such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural by-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide range of pro-oxidants and occurs in vivo during aging and in certain diseases.
The measurement of the end products of lipid peroxidation is one of the most accepted tests to determine oxidative damage. These aldehyde by-products of lipid peroxidation are generally accepted markers of oxidative stress. Thiobarbituric Acid Reactive Substances (TBARS) is a well-established assay for the detection and monitoring of lipid peroxidation. The quick and easy protocol has been modified by researchers in the evaluation of drugs, food, and human and animal tissue samples. MDA forms a 1: 2 adduct with thiobarbituric acid.
The MDA-TBA adduct formed from the reaction of MDA in samples with TBA can be measured colorimetrically or fluorometrically. TBARS levels are determined from a malondialdehyde equivalence standard. The TBARS assay has provided relevant information on the activity of free radicals in disease states and the measurement of the antioxidant characteristics of many compounds. Although the specificity of TBARS for compounds other than MDA has been controversial, the assay remains the most widely used format for monitoring lipid peroxidation.
Lipids with higher degrees of unsaturated bonds produce higher TBARS values. Soluble TBARS interference can be minimized if lipoprotein fractions are acid precipitated from samples first. Biological samples may contain a mixture of reactive substances for thiobarbituric acids, such as hydroperoxides and aldehydes, which increase in response to oxidative stress. If excessively high TBARS values are obtained, a more specific assay such as HPLC should be used.
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.