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A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
Richard
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Nitrotyrosine formation is attributable to presence of reactive oxygen and nitrogen species. Nitration is a really selective course of resulting in particular modification of only some tyrosines in protein molecule. 2D electrophoresis and western blotting strategies coupled with mass spectrometry are frequent strategies utilized in evaluation of proteome.
Right here we describe protocol for evaluation of peroxynitrite-induced protein nitration in remoted mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies in opposition to nitrotyrosine. Optimistic spots are in contrast with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry strategies.
Detection of UVR8 Homodimers and Monomers by Immunoblotting Evaluation in Tomato
The UV RESISTANCE LOCUS 8 (UVR8) is a photoreceptor mediating photomorphogenic responses to UV-B. UVR8 exists as homodimer in crops and UV-B induces dissociation of dimeric UVR8 into monomers to provoke responses. The monomer/dimer standing of UVR8 is reversible and a dynamic photo-equilibrium is established in crops in keeping with the ambient gentle circumstances.
Right here we describe a way to detect UVR8 homodimer and monomer by immunoblotting methodology from tomato (Solanum lycopersicum) crops. The characteristic of this methodology is that protein samples usually are not boiled previous to loading on an SDS-PAGE gel, which permits the detection of UVR8 homodimer and monomers concurrently with a single antibody.
Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative evaluation of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes
The Trichinella genus poses an attention-grabbing puzzle for researchers, having diverged very early within the evolution of the nematodes. The Trichinella spiralis proteome is a cosmopolitan and well-studied mannequin of Trichinella; nevertheless, Trichinella britovi additionally circulates within the sylvatic setting and each species infect people, ensuing within the improvement of trichinellosis. Few experiments have examined the proteins belonging to the T. britovi proteome.
The intention of the current research was to match the protein expression profiles of crude extracts of T. spiralis and T. britovi muscle larvae utilizing a highly-sensitive two-dimensional differential in-gel electrophoresis (2D DIGE) method coupled with 2DE immunoblotting. Chosen immunoreactive protein spots have been then recognized by liquid chromatography coupled with mass spectrometry evaluation (LC-MS/MS), and their operate in Trichinella and the host-parasite interplay was decided by gene ontology evaluation.
Spots frequent to each T. spiralis and T. britovi, spots with completely different expressions between the 2 and spots particular to every species have been labelled with completely different cyanine dyes. In whole, 196 protein spots have been present in each proteomes; of those 165 have been frequent, 23 expressed completely in T. spiralis and eight in T. britovi.
A comparative evaluation of quantity ratio values with Melanie software program confirmed that among the many frequent spots, 9 demonstrated greater expression in T. spiralis, and 17 in T. britovi. LC-MS/MS evaluation of 11 chosen spots recognized 41 proteins with potential antigenic traits: 26 have been particular for T. spiralis, six for T. britovi, and eight have been present in each proteomes.
Gene Ontology evaluation confirmed that the recognized T. spiralis proteins possess hydrolytic endopeptidase, endonuclease and transferase actions. Equally, many of the T. britovi proteins possess catalytic actions, akin to lyase, hydrolase, isomerase and peptidase exercise. The utilized 2D DIGE method visualized Trichinella spp. protein spots with completely different molecular weights or isoelectric level values, in addition to these with completely different expression ranges.
The recognized immunoreactive proteins take part in a number of processes related to host muscle cell invasion and larval adaptation to the host setting. Their reactivity with the host immune system makes them potential candidates for the event of a novel trichinellosis diagnostic check or vaccine in opposition to helminthiasis attributable to T. spiralis or T. britovi.’
Immunoblotting Evaluation of Intracellular PD-L1 Ranges in Interferon-γ-Handled Ovarian Most cancers Cells Stably Transfected with Bcl3 shRNA.
Expression of the immune checkpoint programmed loss of life ligand 1 (PD-L1, CD274) is elevated in lots of sorts of most cancers, together with ovarian most cancers (OC), however the mechanisms that regulate the PD-L1 expression usually are not totally understood. Along with binding to PD-1 on T cells, thus inhibiting T cell-mediated antitumor responses, PD-L1 has additionally tumor-intrinsic results that embody elevated most cancers cell survival and proliferation, and that is perhaps partially mediated by the intracellular PD-L1.
On this chapter, we describe a protocol for the evaluation of the intracellular PD-L1 protein ranges in OC cells by immunoblotting. Our outcomes present that interferon-γ (IFNγ) induces the intracellular ranges of PD-L1 and the proto-oncogene Bcl3 in OC cells.
Nevertheless, the PD-L1 expression is considerably decreased in OC cells stably transfected with Bcl3 shRNA, demonstrating that the IFNγ-induced PD-L1 expression in OC cells is mediated by Bcl3. These information determine the IFNγ-Bcl3-PD-L1 axis as a novel therapeutic goal in OC, and counsel that concentrating on Bcl3 might present a novel technique to manage the PD-L1 expression, and particularly the tumor-intrinsic PD-L1 results mediated by the intracellular PD-L1 in OC cells.
A Fast Extraction Technique for mammalian cell cultures, appropriate for quantitative immunoblotting evaluation of proteins, together with phosphorylated GCN2 and eIF2α.
Many research require the detection and relative quantitation of proteins from cell tradition samples utilizing immunoblotting. Limiting elements are the price of protease inhibitors, the time required to interrupt cells and generate samples, in addition to the excessive threat of protein loss throughout cell breakage procedures. As well as, a typical downside is the viscosity of lysed samples because of the launched genomic DNA.
As a consequence, the DNA must be damaged down previous to denaturing polyacrylamide protein gel electrophoresis (SDS-PAGE), e.g. by passing the pattern by way of a syringe gauge needle, sonication, or DNase therapy.

In a quest to discover a less expensive, quick, and but sturdy process, we discovered that cell lysis, protein denaturation, and DNA fragmentation could be finished in solely two steps: harvesting adopted by a easy non-laborious 2nd step. Equally to many pre-existing cell breakage procedures, in our Fast Protein Extraction (RPE) methodology, proteins liberated from cells are instantly uncovered to a denaturing setting.
Nevertheless, benefits of our methodology are: •No breaking buffer is required, as an alternative proteins are liberated immediately into the denaturing protein loading buffer used for SDS-PAGE. Consequently, our RPE methodology doesn’t require any costly inhibitors.•The RPE methodology doesn’t contain post-lysis centrifugation steps; as an alternative all cell materials is dissolved in the course of the 2nd step, the mixing-heat-treatment step which is new to this methodology.
Acetone, 99.8%, for analysis |
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GK3913-500ML | Glentham Life Sciences | 500 ml | EUR 66 |
Acetone, 99.8%, for analysis |
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GK3913-1 | Glentham Life Sciences | 1 | EUR 38.8 |
Acetone, 99.8%, for analysis |
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GK3913-2500 | Glentham Life Sciences | 2500 | EUR 79.1 |
Acetone, 99.8%, for analysis |
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GK3913-500 | Glentham Life Sciences | 500 | EUR 24.6 |
TISAB for Wine Analysis |
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TISAB-WINE | Scientific Laboratory Supplies | 250ML | EUR 124.8 |
DI PAK FOR NP ANALYSIS |
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CPDI0NJM1 | Scientific Laboratory Supplies | EACH | EUR 342.07 |
Nutrient analysis photometer |
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SPE2110 | Scientific Laboratory Supplies | EACH | EUR 852 |
Hydroxyproline analysis 11-20 samples |
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80062 | Chondrex | Custom service | EUR 167.88 |
Description: Hydroxyproline analysis 11-20 samples |
Hydroxyproline analysis 21-30 samples |
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80063 | Chondrex | Custom service | EUR 162.66 |
Description: Hydroxyproline analysis 21-30 samples |
Hydroxyproline analysis 31-40 samples |
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80064 | Chondrex | Custom service | EUR 159.18 |
Description: Hydroxyproline analysis 31-40 samples |
Collagen analysis, <10 samples |
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80031 | Chondrex | Custom service | EUR 174.84 |
Description: Collagen analysis |
Collagen analysis, >30 samples |
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80033 | Chondrex | Custom service | EUR 162.66 |
Description: Collagen analysis |
Spatular Analysis type 500mm SS |
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DD001810 | Scientific Laboratory Supplies | EACH | EUR 33.6 |
Hydroxyproline analysis <10 samples |
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80061 | Chondrex | Custom service | EUR 171.36 |
Description: Hydroxyproline analysis <10 samples |
EZCellTM Cell Cycle Analysis Kit |
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K920-100 | Biovision | each | EUR 451.2 |
DNA Ploidy Analysis Staining Kit |
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K1439-1 | Biovision | each | EUR 705.6 |
DNA Ploidy Analysis Staining Kit |
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K1439-5 | Biovision | each | EUR 2402.4 |
Peroxide Block for Image Analysis |
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ADA015 | ScyTek Laboratories | 15 ml | EUR 85.2 |
Peroxide Block for Image Analysis |
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ADA500 | ScyTek Laboratories | 500 ml | EUR 152.4 |
Peroxide Block for Image Analysis |
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ADA999 | ScyTek Laboratories | 1000 ml | EUR 188.4 |
Antibody analysis - human >30 samples |
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8005-3 | Chondrex | Custom service | EUR 136.56 |
Description: Antibody analysis - human >30 samples |
Antibody analysis - human <10 samples |
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80051 | Chondrex | Custom service | EUR 147 |
Description: Antibody analysis - human <10 samples |
Sodium dodecyl sulfate, for analysis |
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GX5289-100 | Glentham Life Sciences | 100 | EUR 102.7 |
Sodium dodecyl sulfate, for analysis |
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GX5289-25 | Glentham Life Sciences | 25 | EUR 34.8 |
Exosome size distribution analysis (NTA) |
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S108 | 101Bio | - | EUR 600 |
Antibody analysis - human 10-30 samples |
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8005-2 | Chondrex | Custom service | EUR 140.04 |
Description: Antibody analysis - human 10-30 samples |
Biotin Blocking Kit for Image Analysis |
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BBK030 | ScyTek Laboratories | 30 ml | EUR 142.8 |
Biotin Blocking Kit for Image Analysis |
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BBK120 | ScyTek Laboratories | 120 ml | EUR 258 |
Kjeldahl Analysis Weighing Boat Grade 609 |
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WHA10313032 | Scientific Laboratory Supplies | EACH | EUR 130.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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GK0126-1L | Glentham Life Sciences | 1 l | EUR 136.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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GK0126-2500ML | Glentham Life Sciences | 2500 ml | EUR 246 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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GK0126-1 | Glentham Life Sciences | 1 | EUR 83.1 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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GK0126-2500 | Glentham Life Sciences | 2500 | EUR 174 |
OxiSelect MDA Immunoblot Kit |
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STA-331 | Cell Biolabs | 10 blots | EUR 664.8 |
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Immunoblot Kit provides a specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
ROCK Activity Immunoblot Kit |
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STA-415 | Cell Biolabs | 20 assays | EUR 748.8 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our ROCK Activity Immunoblot Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. |
Attoglow Western Blot Analysis Kit:Blocking Agent |
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K3171250-3 | Biochain | 40 g | EUR 68 |
Blue Feulgen DNA Ploidy Analysis Staining Kit |
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DPK500 | ScyTek Laboratories | 1 kit(s) | EUR 427.2 |
2,3,5-Triphenyltetrazolium chloride, 99%, for analysis |
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GE2454-25 | Glentham Life Sciences | 25 | EUR 113.1 |
2,3,5-Triphenyltetrazolium chloride, 99%, for analysis |
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GE2454-5 | Glentham Life Sciences | 5 | EUR 37.7 |
Antibody analysis - rat, mouse, or human <10 samples |
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80021 | Chondrex | Custom service | EUR 138.3 |
Description: Antibody analysis - rat |
Antibody analysis - rat, mouse, or human >30 samples |
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80023 | Chondrex | Custom service | EUR 127.86 |
Description: Antibody analysis - rat |
Urine Analysis Tube 12ml 16 x 100mm polystyrene |
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G160101 | Scientific Laboratory Supplies | PK1600 | EUR 82.8 |
Attoglow Western Blot Analysis Kit:Binding Buffer 20x |
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K3171250-2 | Biochain | 120 ml | EUR 107 |
Antibody analysis - rat, mouse, or human 10-30 samples |
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80022 | Chondrex | Custom service | EUR 133.08 |
Description: Antibody analysis - rat |
Exo-Flow 2.0 CD9 Analysis Kit (for Serum or Plasma) |
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EXOFLOW2-105A-SP | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma) |
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EXOFLOW2-100A-SP | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD81 Analysis Kit (for Serum or Plasma) |
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EXOFLOW2-110A-SP | SBI | 10 rxn | EUR 705.6 |
C-Series Mouse Inflammation Array Analysis Software |
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S02-AAM-INF-1 | Neuromics | CD | EUR 325.2 |
Antibody analysis - chick, bovine, or porcine <10 samples |
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80011 | Chondrex | Custom service | EUR 129.6 |
Description: Antibody analysis - chick |
Antibody analysis - chick, bovine, or porcine >30 samples |
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80013 | Chondrex | Custom service | EUR 117.42 |
Description: Antibody analysis - chick |
Attoglow Western Blot Analysis Kit: Millennium Enhancer |
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K3171250-1 | Biochain | 100 ml | EUR 130 |
84us Conductivity Solution with certificate of analysis |
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HI-6033 | Scientific Laboratory Supplies | EACH | EUR 38.4 |
OxiSelect Protein Carbonyl Immunoblot Kit |
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STA-308 | Cell Biolabs | 10 blots | EUR 574.8 |
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. |
Checkpoint Kinase Activity Immunoblot Kit |
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STA-413 | Cell Biolabs | 20 assays | EUR 651.6 |
Description: Cell Biolabs? Checkpoint Kinase Activity Immunoblot Kit utilizes recombinant Cdc25C as checkpoint kinase substrate. After incubating the substrate with checkpoint kinase samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated Cdc25C is detected by western blot analysis using an anti-phospho-Cdc25C (Ser216). |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis |
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CHBS-20 | Scientific Laboratory Supplies | PK100 | EUR 15.6 |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis |
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CHBS-20F | Scientific Laboratory Supplies | PK5000 | EUR 699.6 |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis |
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CHBS-20T | Scientific Laboratory Supplies | PK1000 | EUR 147.6 |
Antibody analysis - chick, bovine, or porcine 10-30 samples |
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80012 | Chondrex | Custom service | EUR 122.64 |
Description: Antibody analysis - chick |
Exo-Flow 2.0 CD9 Analysis Kit (for Tissue Culture Media) |
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EXOFLOW2-205A-TC | SBI | 10 rxn | EUR 705.6 |
HR Pooled Cell analysis: 1000ug DR-HRGFP, 10000ug pSce1, 25ug e-GFP, Puromycin |
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DR31000 | TopoGen | 1 | EUR 3423.6 |
Exo-Flow 2.0 CD63 Analysis Kit (for Tissue Culture Media) |
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EXOFLOW2-200A-TC | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD81 Analysis Kit (for Tissue Culture Media) |
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EXOFLOW2-210A-TC | SBI | 10 rxn | EUR 705.6 |
HR Pooled Cell analysis: 500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP, Puromycin |
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DR3500 | TopoGen | 1 | EUR 3174 |
OxiSelect Nitrotyrosine Protein Immunoblot Kit |
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STA-303 | Cell Biolabs | 10 blots | EUR 490.8 |
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine Immunoblot Kit provides a simple method to measure the formation of 3-nitrotyrosine in proteins by Western blot detection. |
OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size |
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STA-308-T | Cell Biolabs | 4 blots | EUR 316.8 |
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. |
Oxidized Protein Immunoblot Control (Carbonyl-BSA) |
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STA-309 | Cell Biolabs | 10 ?g | EUR 289.2 |
Description:
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OxiSelect N-epsilon-(Carboxymethyl) Lysine (CML) Immunoblot Kit |
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STA-313 | Cell Biolabs | 10 blots | EUR 637.2 |
Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Immunoblot kit specifically detects CML formation in protein samples. |
Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2R | Detroit R&D | 1 Kit | EUR 516 |
HR Pooled Cell analysis: 50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP, Puromycin |
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DR3050 | TopoGen | 1 | EUR 2175.6 |
Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2H | Detroit R&D | 1 Kit | EUR 516 |
Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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DD2M | Detroit R&D | 1 Kit | EUR 516 |
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence* |
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15291-100Tests | AAT Bioquest | 100 Tests | EUR 558 |
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence* |
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15291 | AAT Bioquest | 100 Tests | EUR 558 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody |
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K3171120-IV | Biochain | 1200 cm2 | EUR 224 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody |
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K3171250-IV | Biochain | 2500 cm2 | EUR 298 |
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence* |
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15296-100Tests | AAT Bioquest | 100 Tests | EUR 558 |
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence* |
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15296 | AAT Bioquest | 100 Tests | EUR 558 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody |
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K3171120-I | Biochain | 1200 cm2 | EUR 224 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171120-II | Biochain | 1200 cm2 | EUR 224 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody |
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K3171250-I | Biochain | 2500 cm2 | EUR 298 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171250-II | Biochain | 2500 cm2 | EUR 298 |
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-10P | Scientific Laboratory Supplies | PK100 | EUR 10.8 |
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-10PF | Scientific Laboratory Supplies | PK5000 | EUR 484.8 |
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-10PT | Scientific Laboratory Supplies | PK1000 | EUR 103.2 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-20P | Scientific Laboratory Supplies | PK100 | EUR 14.4 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-20PF | Scientific Laboratory Supplies | PK5000 | EUR 645.6 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis |
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CHSV9-20PT | Scientific Laboratory Supplies | PK1000 | EUR 136.8 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody |
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K3171120-III | Biochain | 1200 cm2 | EUR 224 |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody |
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K3171250-III | Biochain | 2500 cm2 | EUR 298 |
2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-10PF | Scientific Laboratory Supplies | PK5000 | EUR 538.8 |
2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-10PT | Scientific Laboratory Supplies | PK1000 | EUR 114 |
2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-20PF | Scientific Laboratory Supplies | PK5000 | EUR 699.6 |
2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-20PT | Scientific Laboratory Supplies | PK1000 | EUR 147.6 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHSC9-35UB | Scientific Laboratory Supplies | PK100 | EUR 26.4 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHSC9-35UBF | Scientific Laboratory Supplies | PK5000 | EUR 1182 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHSC9-35UBT | Scientific Laboratory Supplies | PK1000 | EUR 249.6 |
2mL clear crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-10P | Scientific Laboratory Supplies | PK100 | EUR 12 |
2mL amber crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis |
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CHCV11-20P | Scientific Laboratory Supplies | PK100 | EUR 15.6 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis |
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CHCV20-14 | Scientific Laboratory Supplies | PK100 | EUR 30 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis |
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CHCV20-14F | Scientific Laboratory Supplies | PK5000 | EUR 1226.64 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis |
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CHCV20-14T | Scientific Laboratory Supplies | PK1000 | EUR 284.4 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis |
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CHSC9-35UBS | Scientific Laboratory Supplies | PK100 | EUR 32.4 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis |
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CHSC9-35UBSF | Scientific Laboratory Supplies | PK5000 | EUR 1450.8 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis |
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CHSC9-35UBST | Scientific Laboratory Supplies | PK1000 | EUR 306 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-10 | Scientific Laboratory Supplies | PK100 | EUR 10.8 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-10F | Scientific Laboratory Supplies | PK5000 | EUR 484.8 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-10T | Scientific Laboratory Supplies | PK1000 | EUR 103.2 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-30 | Scientific Laboratory Supplies | PK100 | EUR 22.8 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-30F | Scientific Laboratory Supplies | PK5000 | EUR 1021.2 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis |
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CHCC11-30T | Scientific Laboratory Supplies | PK1000 | EUR 216 |
HeLa Cell RepHR Pooled Cell analysis: 250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP, Puromycinorters to detect DNA Hypomethylating Effectors |
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DR3250 | TopoGen | 1 | EUR 2550 |
CAP SNAP 11MM BLUE POLYETHYLENE INTEGRAL PE SEPTUM THINNED PENETRATION AREA 100/PACK ; Level 1 Everyday Analysis |
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6ERC11PE | Scientific Laboratory Supplies | PK100 | EUR 12 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2 | Scientific Laboratory Supplies | PK100 | EUR 52.8 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2F | Scientific Laboratory Supplies | PK5000 | EUR 2416.8 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2T | Scientific Laboratory Supplies | PK1000 | EUR 510 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-20 | Scientific Laboratory Supplies | PK100 | EUR 18 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-20F | Scientific Laboratory Supplies | PK5000 | EUR 806.4 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-20T | Scientific Laboratory Supplies | PK1000 | EUR 170.4 |
Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-30 | Scientific Laboratory Supplies | PK100 | EUR 19.2 |
Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-30F | Scientific Laboratory Supplies | PK5000 | EUR 860.4 |
Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-30T | Scientific Laboratory Supplies | PK1000 | EUR 181.2 |
Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-40F | Scientific Laboratory Supplies | PK5000 | EUR 1290 |
Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-40T | Scientific Laboratory Supplies | PK1000 | EUR 272.4 |
Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-10 | Scientific Laboratory Supplies | PK100 | EUR 15.6 |
Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-10F | Scientific Laboratory Supplies | PK5000 | EUR 699.6 |
Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-10T | Scientific Laboratory Supplies | PK1000 | EUR 147.6 |
Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1 | Scientific Laboratory Supplies | PK100 | EUR 43.2 |
Silver headspace crimp cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1F | Scientific Laboratory Supplies | PK5000 | EUR 1987.2 |
Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1T | Scientific Laboratory Supplies | PK1000 | EUR 420 |
Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1M | Scientific Laboratory Supplies | PK100 | EUR 66 |
Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1MF | Scientific Laboratory Supplies | PK5000 | EUR 3007.2 |
Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-1MT | Scientific Laboratory Supplies | PK1000 | EUR 634.8 |
Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) - preslit; with AVCS technology ; Level 1 Everyday Analysis |
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CHSC9-40 | Scientific Laboratory Supplies | PK100 | EUR 28.8 |
Kimble Water analysis vial 40ml KG-33 clear glass with attached open top polypropylene Caps PTFE-faced silicone septa |
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VIA2518 | Scientific Laboratory Supplies | PK72 | EUR 256.8 |
Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2MF | Scientific Laboratory Supplies | PK5000 | EUR 4564.8 |
Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2M | Scientific Laboratory Supplies | PK100 | EUR 99.6 |
Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis |
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CHCC20-2MT | Scientific Laboratory Supplies | PK1000 | EUR 961.2 |
AgileSpec? UV-Visible Spectrophotometer, Split Beam (internal double beam), 190-1100nm, analysis software and automatic 6-cell holder included |
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ASP-1100 | ACTGene | each | EUR 8456.4 |
SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service |
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M-PCR-378 | MiTeGen | 500 µl | EUR 72 |
Description: SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service |
AgileSpec™ UV-Visible Spectrophotometer, Split Beam (internal double beam), 190-1100nm, analysis software and automatic 6-cell holder included |
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ASP | ACTGene | 1100 | EUR 6046.81 |
EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service |
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M-PCR-379 | MiTeGen | 500 µl | EUR 72 |
Description: EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service |
Dry Immunofluorescence Analyzer (DIA) |
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DIA00101 | Arthus | 1 set | EUR 3003.6 |
Description: Quantitative test strip fluorescence analyzer. Instrument rental is available at $300/15 days plus shipping & handling. |
Up-converting Phosphor immunoassay analyzer |
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abx095263-1Machine | Abbexa | 1 Machine | EUR 7326 |
Moisture Analyser - 120g . 1mg |
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MOI1002 | Scientific Laboratory Supplies | EACH | EUR 5344.8 |
MB27 Basic Moisture Analyser |
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BAL5550 | Scientific Laboratory Supplies | EACH | EUR 2150.4 |
Infrared Moisture Analyser |
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MOI3050 | Scientific Laboratory Supplies | EACH | EUR 2856 |
Analyser slider w rotating mount |
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MIC1584 | Scientific Laboratory Supplies | EACH | EUR 167.12 |
HiQTM Immuno Block |
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I3211-010 | GenDepot | 100ml | EUR 160.8 |
HiQTM Immuno Block |
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I3211-050 | GenDepot | 500ml | EUR 319.2 |
Sartorius MA37-1 Moisture Analyser |
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BAL1150 | Scientific Laboratory Supplies | EACH | EUR 4957.2 |
Purite Analyst 40/80/160 Annual Consumable Pack |
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WAT1090 | Scientific Laboratory Supplies | EACH | EUR 990 |
Dinitrophenol (2,4-DNP), >98% pure (protein analyses grade) |
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DNP90-N-1 | Alpha Diagnostics | 1 g | EUR 489.6 |
Suez Purite Analyst 320/Purewater 300 Annual Consumable Pack |
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WAT1094 | Scientific Laboratory Supplies | EACH | EUR 1616.4 |
Free Protein Sequence Analyses (PSSA) for antigenicity Order form |
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PSSA-1 | Alpha Diagnostics | 1 | Ask for price |
Rat Anti immunobulin Binding Protein antibody ELISA kit |
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E02B0030-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti immunobulin Binding Protein antibody ELISA kit |
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E02B0030-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti immunobulin Binding Protein antibody ELISA kit |
|||
E02B0030-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti immunobulin Binding Protein antibody ELISA kit |
|||
E07B0030-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti immunobulin Binding Protein antibody ELISA kit |
|||
E07B0030-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti immunobulin Binding Protein antibody ELISA kit |
|||
E07B0030-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti immunobulin Binding Protein antibody ELISA kit |
|||
E08B0030-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti immunobulin Binding Protein antibody ELISA kit |
|||
E08B0030-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti immunobulin Binding Protein antibody ELISA kit |
|||
E08B0030-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti immunobulin Binding Protein antibody ELISA kit |
|||
E06B0030-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti immunobulin Binding Protein antibody ELISA kit |
|||
E06B0030-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti immunobulin Binding Protein antibody ELISA kit |
|||
E06B0030-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
×
This prevents potential protein loss which will happen throughout centrifugation. As well as, this 2nd step concurrently shears the genomic DNA, making a further step for DNA fragmentation pointless.•The generated samples are appropriate for high-quality quantitative immunoblotting. With our RPE methodology we efficiently quantified the phosphorylated types of protein kinase GCN2 and its substrate eIF2α. In actual fact, the western alerts have been stronger and with much less background, as in comparison with samples generated with a pre-existing methodology.
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