immunoblotting analysis, A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

Nitrotyrosine formation is attributable to presence of reactive oxygen and nitrogen species. Nitration is a really selective course of resulting in particular modification of only some tyrosines in protein molecule. 2D electrophoresis and western blotting strategies coupled with mass spectrometry are frequent strategies utilized in evaluation of proteome.
Right here we describe protocol for evaluation of peroxynitrite-induced protein nitration in remoted mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies in opposition to nitrotyrosine. Optimistic spots are in contrast with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry strategies.

Detection of UVR8 Homodimers and Monomers by Immunoblotting Evaluation in Tomato

The UV RESISTANCE LOCUS 8 (UVR8) is a photoreceptor mediating photomorphogenic responses to UV-B. UVR8 exists as homodimer in crops and UV-B induces dissociation of dimeric UVR8 into monomers to provoke responses. The monomer/dimer standing of UVR8 is reversible and a dynamic photo-equilibrium is established in crops in keeping with the ambient gentle circumstances.
Right here we describe a way to detect UVR8 homodimer and monomer by immunoblotting methodology from tomato (Solanum lycopersicum) crops. The characteristic of this methodology is that protein samples usually are not boiled previous to loading on an SDS-PAGE gel, which permits the detection of UVR8 homodimer and monomers concurrently with a single antibody.

Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative evaluation of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes

The Trichinella genus poses an attention-grabbing puzzle for researchers, having diverged very early within the evolution of the nematodes. The Trichinella spiralis proteome is a cosmopolitan and well-studied mannequin of Trichinella; nevertheless, Trichinella britovi additionally circulates within the sylvatic setting and each species infect people, ensuing within the improvement of trichinellosis. Few experiments have examined the proteins belonging to the T. britovi proteome.
The intention of the current research was to match the protein expression profiles of crude extracts of T. spiralis and T. britovi muscle larvae utilizing a highly-sensitive two-dimensional differential in-gel electrophoresis (2D DIGE) method coupled with 2DE immunoblotting. Chosen immunoreactive protein spots have been then recognized by liquid chromatography coupled with mass spectrometry evaluation (LC-MS/MS), and their operate in Trichinella and the host-parasite interplay was decided by gene ontology evaluation.
Spots frequent to each T. spiralis and T. britovi, spots with completely different expressions between the 2 and spots particular to every species have been labelled with completely different cyanine dyes. In whole, 196 protein spots have been present in each proteomes; of those 165 have been frequent, 23 expressed completely in T. spiralis and eight in T. britovi.
A comparative evaluation of quantity ratio values with Melanie software program confirmed that among the many frequent spots, 9 demonstrated greater expression in T. spiralis, and 17 in T. britovi. LC-MS/MS evaluation of 11 chosen spots recognized 41 proteins with potential antigenic traits: 26 have been particular for T. spiralis, six for T. britovi, and eight have been present in each proteomes.
Gene Ontology evaluation confirmed that the recognized T. spiralis proteins possess hydrolytic endopeptidase, endonuclease and transferase actions. Equally, many of the T. britovi proteins possess catalytic actions, akin to lyase, hydrolase, isomerase and peptidase exercise. The utilized 2D DIGE method visualized Trichinella spp. protein spots with completely different molecular weights or isoelectric level values, in addition to these with completely different expression ranges.
The recognized immunoreactive proteins take part in a number of processes related to host muscle cell invasion and larval adaptation to the host setting. Their reactivity with the host immune system makes them potential candidates for the event of a novel trichinellosis diagnostic check or vaccine in opposition to helminthiasis attributable to T. spiralis or T. britovi.’

Immunoblotting Evaluation of Intracellular PD-L1 Ranges in Interferon-γ-Handled Ovarian Most cancers Cells Stably Transfected with Bcl3 shRNA.

Expression of the immune checkpoint programmed loss of life ligand 1 (PD-L1, CD274) is elevated in lots of sorts of most cancers, together with ovarian most cancers (OC), however the mechanisms that regulate the PD-L1 expression usually are not totally understood. Along with binding to PD-1 on T cells, thus inhibiting T cell-mediated antitumor responses, PD-L1 has additionally tumor-intrinsic results that embody elevated most cancers cell survival and proliferation, and that is perhaps partially mediated by the intracellular PD-L1.
On this chapter, we describe a protocol for the evaluation of the intracellular PD-L1 protein ranges in OC cells by immunoblotting. Our outcomes present that interferon-γ (IFNγ) induces the intracellular ranges of PD-L1 and the proto-oncogene Bcl3 in OC cells.
Nevertheless, the PD-L1 expression is considerably decreased in OC cells stably transfected with Bcl3 shRNA, demonstrating that the IFNγ-induced PD-L1 expression in OC cells is mediated by Bcl3. These information determine the IFNγ-Bcl3-PD-L1 axis as a novel therapeutic goal in OC, and counsel that concentrating on Bcl3 might present a novel technique to manage the PD-L1 expression, and particularly the tumor-intrinsic PD-L1 results mediated by the intracellular PD-L1 in OC cells.

A Fast Extraction Technique for mammalian cell cultures, appropriate for quantitative immunoblotting evaluation of proteins, together with phosphorylated GCN2 and eIF2α.

Many research require the detection and relative quantitation of proteins from cell tradition samples utilizing immunoblotting. Limiting elements are the price of protease inhibitors, the time required to interrupt cells and generate samples, in addition to the excessive threat of protein loss throughout cell breakage procedures. As well as, a typical downside is the viscosity of lysed samples because of the launched genomic DNA.
As a consequence, the DNA must be damaged down previous to denaturing polyacrylamide protein gel electrophoresis (SDS-PAGE), e.g. by passing the pattern by way of a syringe gauge needle, sonication, or DNase therapy.
immunoblotting analysis, A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
In a quest to discover a less expensive, quick, and but sturdy process, we discovered that cell lysis, protein denaturation, and DNA fragmentation could be finished in solely two steps: harvesting adopted by a easy non-laborious 2nd step. Equally to many pre-existing cell breakage procedures, in our Fast Protein Extraction (RPE) methodology, proteins liberated from cells are instantly uncovered to a denaturing setting.
Nevertheless, benefits of our methodology are: •No breaking buffer is required, as an alternative proteins are liberated immediately into the denaturing protein loading buffer used for SDS-PAGE. Consequently, our RPE methodology doesn’t require any costly inhibitors.•The RPE methodology doesn’t contain post-lysis centrifugation steps; as an alternative all cell materials is dissolved in the course of the 2nd step, the mixing-heat-treatment step which is new to this methodology.

Rtg Rapid Analysis Kit

27950201 EACH
EUR 1110.36

TISAB for Wine Analysis

TISAB-WINE 250ML
EUR 101.46

Collagen analysis, 10-30 samples

80032 Custom service
EUR 169.62
Description: Collagen analysis

Antibody analysis - human >30 samples

8005-3 Custom service
EUR 136.56
Description: Antibody analysis - human >30 samples

Antibody analysis - human <10 samples

80051 Custom service
EUR 147
Description: Antibody analysis - human <10 samples

Hydroxyproline analysis 11-20 samples

80062 Custom service
EUR 167.88
Description: Hydroxyproline analysis 11-20 samples

Hydroxyproline analysis 21-30 samples

80063 Custom service
EUR 162.66
Description: Hydroxyproline analysis 21-30 samples

Hydroxyproline analysis 31-40 samples

80064 Custom service
EUR 159.18
Description: Hydroxyproline analysis 31-40 samples

Peroxide Block for Image Analysis

ADA015 15 ml
EUR 85.2

Peroxide Block for Image Analysis

ADA500 500 ml
EUR 152.4

Peroxide Block for Image Analysis

ADA999 1000 ml
EUR 188.4

EZCellTM Cell Cycle Analysis Kit

K920-100
EUR 451.2

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2402.4

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1L 1 l
EUR 136.8

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500ML 2500 ml
EUR 246

DI PAK FOR NP ANALYSIS

CPDI0NJM1 EACH
EUR 253.08

Spatular Analysis type 500mm SS

DD001810 EACH
EUR 27.36

Antibody analysis - human 10-30 samples

8005-2 Custom service
EUR 140.04
Description: Antibody analysis - human 10-30 samples

Biotin Blocking Kit for Image Analysis

BBK030 30 ml
EUR 142.8

Biotin Blocking Kit for Image Analysis

BBK120 120 ml
EUR 258

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 130.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Kjeldahl Analysis Weighing Boat Grade 609

WHA10313032 EACH
EUR 109.44

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 427.2

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 195.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 171.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

C-Series Mouse Inflammation Array Analysis Software

S02-AAM-INF-1 CD
EUR 325.2

84us Conductivity Solution with certificate of analysis

HI-6033 EACH
EUR 29.64

Antibody analysis - chick, bovine, or porcine <10 samples

80011 Custom service
EUR 129.6
Description: Antibody analysis - chick

Antibody analysis - chick, bovine, or porcine >30 samples

80013 Custom service
EUR 117.42
Description: Antibody analysis - chick

Antibody analysis - rat, mouse, or human <10 samples

80021 Custom service
EUR 138.3
Description: Antibody analysis - rat

Antibody analysis - rat, mouse, or human >30 samples

80023 Custom service
EUR 127.86
Description: Antibody analysis - rat

Urine Analysis Tube 12ml 16 x 100mm polystyrene

G160101 PK1600
EUR 78.66

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis

CHBS-20 PK100
EUR 14.15

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis

CHBS-20F PK5000
EUR 638.4

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis

CHBS-20T PK1000
EUR 134.52

Antibody analysis - chick, bovine, or porcine 10-30 samples

80012 Custom service
EUR 122.64
Description: Antibody analysis - chick

Antibody analysis - rat, mouse, or human 10-30 samples

80022 Custom service
EUR 133.08
Description: Antibody analysis - rat

Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit

15291 100 Tests
EUR 576

Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma)

EXOFLOW2-100A-SP 10 rxn
EUR 705.6

Exo-Flow 2.0 CD9 Analysis Kit (for Serum or Plasma)

EXOFLOW2-105A-SP 10 rxn
EUR 705.6

Exo-Flow 2.0 CD81 Analysis Kit (for Serum or Plasma)

EXOFLOW2-110A-SP 10 rxn
EUR 705.6

Exo-Flow 2.0 CD63 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-200A-TC 10 rxn
EUR 705.6

Exo-Flow 2.0 CD9 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-205A-TC 10 rxn
EUR 705.6

Exo-Flow 2.0 CD81 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-210A-TC 10 rxn
EUR 705.6

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 516

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 516

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 516

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody

K3171120-I 1200 cm2
EUR 295.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171120-II 1200 cm2
EUR 295.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171120-III 1200 cm2
EUR 295.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171120-IV 1200 cm2
EUR 295.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody

K3171250-I 2500 cm2
EUR 372
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171250-II 2500 cm2
EUR 372
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171250-III 2500 cm2
EUR 372
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171250-IV 2500 cm2
EUR 372
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

1.5mL clear screw PP vial (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-1PP PK100
EUR 31.58

1.5mL clear screw PP vial (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-1PPF PK5000
EUR 1422.72

1.5mL clear screw PP vial (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-1PPT PK1000
EUR 299.82

HR Pooled Cell analysis:  50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP, Puromycin

DR3050 1
EUR 2175.6

HR Pooled Cell analysis:  1000ug DR-HRGFP, 10000ug pSce1, 25ug e-GFP, Puromycin

DR31000 1
EUR 3423.6

HR Pooled Cell analysis:  500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP, Puromycin

DR3500 1
EUR 3174

Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence*

15296 100 Tests
EUR 558

0.3mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-03PPC PK100
EUR 20.66

0.3mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-03PPCF PK5000
EUR 932.52

0.3mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-03PPCT PK1000
EUR 197.22

0.8mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPC PK100
EUR 20.66

0.8mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPCF PK5000
EUR 932.52

0.8mL clear snap PP vial with fixed conical insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPCT PK1000
EUR 197.22

0.8mL clear snap PP vial with fixed flat bottom insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPFB PK100
EUR 22.91

0.8mL clear snap PP vial with fixed flat bottom insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPFBF PK5000
EUR 1030.56

0.8mL clear snap PP vial with fixed flat bottom insert (11mm thread) ; Level 1 Everyday Analysis

6ERV11-08PPFBT PK1000
EUR 217.74

0.4mL clear screw PP vial with fixed conical insert (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-04PP PK100
EUR 21.74

0.4mL clear screw PP vial with fixed conical insert (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-04PPF PK5000
EUR 981.54

0.4mL clear screw PP vial with fixed conical insert (9mm short thread) ; Level 1 Everyday Analysis

6ESV9-04PPT PK1000
EUR 207.48

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-10P PK100
EUR 9.53

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-10PF PK5000
EUR 442.32

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-10PT PK1000
EUR 93.48

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-20P PK100
EUR 12.21

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-20PF PK5000
EUR 589.38

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis

CHSV9-20PT PK1000
EUR 124.26

CAP SNAP 11MM BLUE POLYETHYLENE INTEGRAL PE SEPTUM THINNED PENETRATION AREA 100/PACK ; Level 1 Everyday Analysis

6ERC11PE PK100
EUR 10.89

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis

CHSC9-35UB PK100
EUR 23.37

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis

CHSC9-35UBF PK5000
EUR 1079.58

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis

CHSC9-35UBT PK1000
EUR 228

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2 PK100
EUR 47.65

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2F PK5000
EUR 2207.04

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2T PK1000
EUR 465.12

2mL clear crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-10P PK100
EUR 10.58

2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-10PF PK5000
EUR 491.34

2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-10PT PK1000
EUR 103.74

2mL amber crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-20P PK100
EUR 13.76

2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-20PF PK5000
EUR 638.4

2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis

CHCV11-20PT PK1000
EUR 134.52

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis

CHSC9-35UBS PK100
EUR 28.61

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis

CHSC9-35UBSF PK5000
EUR 1324.68

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis

CHSC9-35UBST PK1000
EUR 279.3

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis

CHCC11-10 PK100
EUR 9.53

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis

CHCC11-10F PK5000
EUR 442.32

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis

CHCC11-10T PK1000
EUR 93.48

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis

CHCC11-30 PK100
EUR 20.11

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis

CHCC11-30F PK5000
EUR 932.52

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis

CHCC11-30T PK1000
EUR 197.22

Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1 PK100
EUR 39.22

Silver headspace crimp cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1F PK5000
EUR 1814.88

Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1T PK1000
EUR 383.04

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis

CHCV20-14 PK100
EUR 26.56

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis

CHCV20-14F PK5000
EUR 1226.64

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis

CHCV20-14T PK1000
EUR 258.78

AgileSpec? UV-Visible Spectrophotometer, Split Beam (internal double beam), 190-1100nm, analysis software and automatic 6-cell holder included

ASP-1100
EUR 8456.4

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-20 PK100
EUR 15.88

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-20F PK5000
EUR 736.44

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-20T PK1000
EUR 155.04

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-30 PK100
EUR 16.93

Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-30F PK5000
EUR 785.46

Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-30T PK1000
EUR 165.3

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-40F PK5000
EUR 1177.62

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-40T PK1000
EUR 248.52

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1M PK100
EUR 59.28

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1MF PK5000
EUR 2746.26

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-1MT PK1000
EUR 579.12

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2MF PK5000
EUR 4168.98

Kimble Water analysis vial 40ml KG-33 clear glass with attached open top polypropylene Caps PTFE-faced silicone septa

VIA2518 PK72
EUR 221.16

HeLa Cell RepHR Pooled Cell analysis: 250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP, Puromycinorters to detect DNA Hypomethylating Effectors

DR3250 1
EUR 2550

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-10 PK100
EUR 13.76

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-10F PK5000
EUR 638.4

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis

CHSC9-10T PK1000
EUR 134.52

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) - preslit; with AVCS technology ; Level 1 Everyday Analysis

CHSC9-40 PK100
EUR 25.42

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2M PK100
EUR 90.06

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis

CHCC20-2MT PK1000
EUR 877.8

SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

M-PCR-378 500 µl
EUR 63.63
Description: SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service

M-PCR-379 500 µl
EUR 63.63
Description: EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service
This prevents potential protein loss which will happen throughout centrifugation. As well as, this 2nd step concurrently shears the genomic DNA, making a further step for DNA fragmentation pointless.•The generated samples are appropriate for high-quality quantitative immunoblotting. With our RPE methodology we efficiently quantified the phosphorylated types of protein kinase GCN2 and its substrate eIF2α. In actual fact, the western alerts have been stronger and with much less background, as in comparison with samples generated with a pre-existing methodology.

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