immunoblotting analysis, A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

Nitrotyrosine formation is attributable to presence of reactive oxygen and nitrogen species. Nitration is a really selective course of resulting in particular modification of only some tyrosines in protein molecule. 2D electrophoresis and western blotting strategies coupled with mass spectrometry are frequent strategies utilized in evaluation of proteome.
Right here we describe protocol for evaluation of peroxynitrite-induced protein nitration in remoted mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies in opposition to nitrotyrosine. Optimistic spots are in contrast with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry strategies.

Detection of UVR8 Homodimers and Monomers by Immunoblotting Evaluation in Tomato

The UV RESISTANCE LOCUS 8 (UVR8) is a photoreceptor mediating photomorphogenic responses to UV-B. UVR8 exists as homodimer in crops and UV-B induces dissociation of dimeric UVR8 into monomers to provoke responses. The monomer/dimer standing of UVR8 is reversible and a dynamic photo-equilibrium is established in crops in keeping with the ambient gentle circumstances.
Right here we describe a way to detect UVR8 homodimer and monomer by immunoblotting methodology from tomato (Solanum lycopersicum) crops. The characteristic of this methodology is that protein samples usually are not boiled previous to loading on an SDS-PAGE gel, which permits the detection of UVR8 homodimer and monomers concurrently with a single antibody.

Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative evaluation of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes

The Trichinella genus poses an attention-grabbing puzzle for researchers, having diverged very early within the evolution of the nematodes. The Trichinella spiralis proteome is a cosmopolitan and well-studied mannequin of Trichinella; nevertheless, Trichinella britovi additionally circulates within the sylvatic setting and each species infect people, ensuing within the improvement of trichinellosis. Few experiments have examined the proteins belonging to the T. britovi proteome.
The intention of the current research was to match the protein expression profiles of crude extracts of T. spiralis and T. britovi muscle larvae utilizing a highly-sensitive two-dimensional differential in-gel electrophoresis (2D DIGE) method coupled with 2DE immunoblotting. Chosen immunoreactive protein spots have been then recognized by liquid chromatography coupled with mass spectrometry evaluation (LC-MS/MS), and their operate in Trichinella and the host-parasite interplay was decided by gene ontology evaluation.
Spots frequent to each T. spiralis and T. britovi, spots with completely different expressions between the 2 and spots particular to every species have been labelled with completely different cyanine dyes. In whole, 196 protein spots have been present in each proteomes; of those 165 have been frequent, 23 expressed completely in T. spiralis and eight in T. britovi.
A comparative evaluation of quantity ratio values with Melanie software program confirmed that among the many frequent spots, 9 demonstrated greater expression in T. spiralis, and 17 in T. britovi. LC-MS/MS evaluation of 11 chosen spots recognized 41 proteins with potential antigenic traits: 26 have been particular for T. spiralis, six for T. britovi, and eight have been present in each proteomes.
Gene Ontology evaluation confirmed that the recognized T. spiralis proteins possess hydrolytic endopeptidase, endonuclease and transferase actions. Equally, many of the T. britovi proteins possess catalytic actions, akin to lyase, hydrolase, isomerase and peptidase exercise. The utilized 2D DIGE method visualized Trichinella spp. protein spots with completely different molecular weights or isoelectric level values, in addition to these with completely different expression ranges.
The recognized immunoreactive proteins take part in a number of processes related to host muscle cell invasion and larval adaptation to the host setting. Their reactivity with the host immune system makes them potential candidates for the event of a novel trichinellosis diagnostic check or vaccine in opposition to helminthiasis attributable to T. spiralis or T. britovi.’

Immunoblotting Evaluation of Intracellular PD-L1 Ranges in Interferon-γ-Handled Ovarian Most cancers Cells Stably Transfected with Bcl3 shRNA.

Expression of the immune checkpoint programmed loss of life ligand 1 (PD-L1, CD274) is elevated in lots of sorts of most cancers, together with ovarian most cancers (OC), however the mechanisms that regulate the PD-L1 expression usually are not totally understood. Along with binding to PD-1 on T cells, thus inhibiting T cell-mediated antitumor responses, PD-L1 has additionally tumor-intrinsic results that embody elevated most cancers cell survival and proliferation, and that is perhaps partially mediated by the intracellular PD-L1.
On this chapter, we describe a protocol for the evaluation of the intracellular PD-L1 protein ranges in OC cells by immunoblotting. Our outcomes present that interferon-γ (IFNγ) induces the intracellular ranges of PD-L1 and the proto-oncogene Bcl3 in OC cells.
Nevertheless, the PD-L1 expression is considerably decreased in OC cells stably transfected with Bcl3 shRNA, demonstrating that the IFNγ-induced PD-L1 expression in OC cells is mediated by Bcl3. These information determine the IFNγ-Bcl3-PD-L1 axis as a novel therapeutic goal in OC, and counsel that concentrating on Bcl3 might present a novel technique to manage the PD-L1 expression, and particularly the tumor-intrinsic PD-L1 results mediated by the intracellular PD-L1 in OC cells.

A Fast Extraction Technique for mammalian cell cultures, appropriate for quantitative immunoblotting evaluation of proteins, together with phosphorylated GCN2 and eIF2α.

Many research require the detection and relative quantitation of proteins from cell tradition samples utilizing immunoblotting. Limiting elements are the price of protease inhibitors, the time required to interrupt cells and generate samples, in addition to the excessive threat of protein loss throughout cell breakage procedures. As well as, a typical downside is the viscosity of lysed samples because of the launched genomic DNA.
As a consequence, the DNA must be damaged down previous to denaturing polyacrylamide protein gel electrophoresis (SDS-PAGE), e.g. by passing the pattern by way of a syringe gauge needle, sonication, or DNase therapy.
immunoblotting analysis, A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
In a quest to discover a less expensive, quick, and but sturdy process, we discovered that cell lysis, protein denaturation, and DNA fragmentation could be finished in solely two steps: harvesting adopted by a easy non-laborious 2nd step. Equally to many pre-existing cell breakage procedures, in our Fast Protein Extraction (RPE) methodology, proteins liberated from cells are instantly uncovered to a denaturing setting.
Nevertheless, benefits of our methodology are: •No breaking buffer is required, as an alternative proteins are liberated immediately into the denaturing protein loading buffer used for SDS-PAGE. Consequently, our RPE methodology doesn’t require any costly inhibitors.•The RPE methodology doesn’t contain post-lysis centrifugation steps; as an alternative all cell materials is dissolved in the course of the 2nd step, the mixing-heat-treatment step which is new to this methodology.

Acetone, 99.8%, for analysis

GK3913-2500 2500
EUR 79.1

Acetone, 99.8%, for analysis

GK3913-2500ML 2500 ml
EUR 132

Acetone, 99.8%, for analysis

GK3913-500 500
EUR 24.6

Acetone, 99.8%, for analysis

GK3913-500ML 500 ml
EUR 66

Hydroxyproline analysis 11-20 samples

80062 Custom service
EUR 167.88
Description: Hydroxyproline analysis 11-20 samples

Hydroxyproline analysis 31-40 samples

80064 Custom service
EUR 159.18
Description: Hydroxyproline analysis 31-40 samples

Collagen analysis, <10 samples

80031 Custom service
EUR 174.84
Description: Collagen analysis

Collagen analysis, >30 samples

80033 Custom service
EUR 162.66
Description: Collagen analysis

Rtg Rapid Analysis Kit - EACH

27950201 EACH
EUR 1482.3

ANA 9 Line Immunoblot 08 ELISA kit

55R-ORG710/08 8 Tests
EUR 165
Description: ELISA kit for the detection of ANA 9 Line Immunoblot 08 in the research laboratory

ANA 9 Line Immunoblot 16 ELISA kit

55R-ORG710/16 16 Tests
EUR 300
Description: ELISA kit for the detection of ANA 9 Line Immunoblot 16 in the research laboratory

Rtg Rapd Analysis Beads - EACH

27950001 EACH
EUR 1074.6

DI PAK FOR NP ANALYSIS - EACH

CPDI0NJM1 EACH
EUR 384.83

Nutrient analysis photometer - EACH

SPE2110 EACH
EUR 958.5

Hydroxyproline analysis <10 samples

80061 Custom service
EUR 171.36
Description: Hydroxyproline analysis <10 samples

EZCellTM Cell Cycle Analysis Kit

K920-100 each
EUR 451.2

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Peroxide Block for Image Analysis

ADA015 15 ml
EUR 16.72

Peroxide Block for Image Analysis

ADA500 500 ml
EUR 61.72

Peroxide Block for Image Analysis

ADA999 1000 ml
EUR 86.15

Antibody analysis - human >30 samples

8005-3 Custom service
EUR 136.56
Description: Antibody analysis - human >30 samples

Antibody analysis - human <10 samples

80051 Custom service
EUR 147
Description: Antibody analysis - human <10 samples

Sodium dodecyl sulfate, for analysis

GX5289-100 100
EUR 102.7

Sodium dodecyl sulfate, for analysis

GX5289-25 25
EUR 34.8

Spatular Analysis type 500mm SS - EACH

DD001810 EACH
EUR 37.8

Exosome size distribution analysis (NTA)

S108 -
EUR 600

Antibody analysis - human 10-30 samples

8005-2 Custom service
EUR 140.04
Description: Antibody analysis - human 10-30 samples

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1 1
EUR 83.1

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-1L 1 l
EUR 136.8

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500 2500
EUR 174

Tetrahydrofuran, 99.9%, for analysis, unstabilised

GK0126-2500ML 2500 ml
EUR 246

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 78

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 245.58

Kjeldahl Analysis Weighing Boat Grade 609 - PK100

WHA10313032 EACH
EUR 147.15

2,3,5-Triphenyltetrazolium chloride, 99%, for analysis

GE2454-25 25
EUR 113.1

2,3,5-Triphenyltetrazolium chloride, 99%, for analysis

GE2454-5 5
EUR 37.7

Antibody analysis - rat, mouse, or human <10 samples

80021 Custom service
EUR 138.3
Description: Antibody analysis - rat

Antibody analysis - rat, mouse, or human >30 samples

80023 Custom service
EUR 127.86
Description: Antibody analysis - rat

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 123

Rubella Virus IgG Immunoblot

GWB-521231 1x96 Assays Ask for price

OxiSelect MDA Immunoblot Kit

STA-331 10 blots
EUR 664.8
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Immunoblot Kit provides a specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

ROCK Activity Immunoblot Kit

STA-415 20 assays
EUR 525

Antibody analysis - rat, mouse, or human 10-30 samples

80022 Custom service
EUR 133.08
Description: Antibody analysis - rat

Exo-Flow 2.0 CD9 Analysis Kit (for Serum or Plasma)

EXOFLOW2-105A-SP 10 rxn
EUR 705.6

C-Series Mouse Inflammation Array Analysis Software

S02-AAM-INF-1 CD
EUR 325.2

Antibody analysis - chick, bovine, or porcine <10 samples

80011 Custom service
EUR 129.6
Description: Antibody analysis - chick

Antibody analysis - chick, bovine, or porcine >30 samples

80013 Custom service
EUR 117.42
Description: Antibody analysis - chick

Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma)

EXOFLOW2-100A-SP 10 rxn
EUR 705.6

Exo-Flow 2.0 CD81 Analysis Kit (for Serum or Plasma)

EXOFLOW2-110A-SP 10 rxn
EUR 705.6

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 150

Urine Analysis Tube 12ml 16 x 100mm polystyrene - PK1600

G160101 PK1600
EUR 93.15

Antibody analysis - chick, bovine, or porcine 10-30 samples

80012 Custom service
EUR 122.64
Description: Antibody analysis - chick

Exo-Flow 2.0 CD9 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-205A-TC 10 rxn
EUR 705.6

HR Pooled Cell analysis:  1000ug DR-HRGFP, 10000ug pSce1, 25ug e-GFP, Puromycin

DR31000 1
EUR 3423.6

Exo-Flow 2.0 CD63 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-200A-TC 10 rxn
EUR 705.6

Exo-Flow 2.0 CD81 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-210A-TC 10 rxn
EUR 705.6

84us Conductivity Solution with certificate of analysis - EACH

HI-6033 EACH
EUR 43.2

HR Pooled Cell analysis:  500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP, Puromycin

DR3500 1
EUR 3174

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK100

CHBS-20 PK100
EUR 17.55

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK5000

CHBS-20F PK5000
EUR 787.05

Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK1000

CHBS-20T PK1000
EUR 166.05

OxiSelect™ Protein Carbonyl Immunoblot Kit

STA-308 10 blots
EUR 410

Checkpoint Kinase Activity Immunoblot Kit

STA-413 20 assays
EUR 651.6
Description: Cell Biolabs? Checkpoint Kinase Activity Immunoblot Kit utilizes recombinant Cdc25C as checkpoint kinase substrate. After incubating the substrate with checkpoint kinase samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated Cdc25C is detected by western blot analysis using an anti-phospho-Cdc25C (Ser216).

Exo-FACS ready to use kit for analysis of human SERUM exosomes

HBM-FACS-PES KIT
EUR 272.16

Exo-FACS ready to use kit for analysis of human URINE exosomes

HBM-FACS-PEU KIT
EUR 347.76

Exo-FACS ready to use kit for analysis of human PLASMA exosomes

HBM-FACS-PEP KIT
EUR 272.16

OxiSelect Nitrotyrosine Protein Immunoblot Kit

STA-303 10 blots
EUR 490.8
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine Immunoblot Kit provides a simple method to measure the formation of 3-nitrotyrosine in proteins by Western blot detection.

OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size

STA-308-T 4 blots
EUR 316.8
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints.

Oxidized Protein Immunoblot Control (Carbonyl-BSA)

STA-309 10 ?g
EUR 170

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 550

HR Pooled Cell analysis:  50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP, Puromycin

DR3050 1
EUR 2175.6

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 550

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 550

OxiSelect N-epsilon-(Carboxymethyl) Lysine (CML) Immunoblot Kit

STA-313 10 blots
EUR 637.2
Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Immunoblot kit specifically detects CML formation in protein samples.

Mikrogen Diagnostik recomBlot Rubella IgG Immunoblot Test Kit

4902 20 Tests
EUR 660

Exo-FACS ready to use kit for analysis of exosomes markers from cell media

HBM-FACS-C KIT
EUR 347.76

Exo-FACS ready to use kit for analysis of exosomes markers from MSC exosomes

HBM-FACS-MSC KIT
EUR 393.12

Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence*

15291 100 Tests
EUR 570
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.

Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence*

15291-100Tests 100 Tests
EUR 558
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.

Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence*

15296 100 Tests
EUR 570
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.

Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence*

15296-100Tests 100 Tests
EUR 558
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171120-IV 1200 cm2
EUR 258

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171250-IV 2500 cm2
EUR 343

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody

K3171120-I 1200 cm2
EUR 258

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171120-II 1200 cm2
EUR 258

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody

K3171250-I 2500 cm2
EUR 343

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171250-II 2500 cm2
EUR 343

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171120-III 1200 cm2
EUR 258

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171250-III 2500 cm2
EUR 343

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK100

CHSV9-10P PK100
EUR 12.15

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK5000

CHSV9-10PF PK5000
EUR 545.4

2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK1000

CHSV9-10PT PK1000
EUR 116.1

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK100

CHSV9-20P PK100
EUR 16.2

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK5000

CHSV9-20PF PK5000
EUR 726.3

2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK1000

CHSV9-20PT PK1000
EUR 153.9

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis

CHCV20-14F PK5000
EUR 1226.64

2mL clear crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK100

CHCV11-10P PK100
EUR 13.5

2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK5000

CHCV11-10PF PK5000
EUR 606.15

2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK1000

CHCV11-10PT PK1000
EUR 128.25

2mL amber crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK100

CHCV11-20P PK100
EUR 17.55

2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK5000

CHCV11-20PF PK5000
EUR 787.05

2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK1000

CHCV11-20PT PK1000
EUR 166.05

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHSC9-35UB PK100
EUR 29.7

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHSC9-35UBF PK5000
EUR 1329.75

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHSC9-35UBT PK1000
EUR 280.8

HeLa Cell RepHR Pooled Cell analysis: 250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP, Puromycinorters to detect DNA Hypomethylating Effectors

DR3250 1
EUR 2550

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis - PK100

CHCV20-14 PK100
EUR 33.75

20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis - PK1000

CHCV20-14T PK1000
EUR 319.95

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK100

CHSC9-35UBS PK100
EUR 36.45

CAP SNAP 11MM BLUE POLYETHYLENE INTEGRAL PE SEPTUM THINNED PENETRATION AREA 100/PACK ; Level 1 Everyday Analysis - PK100

6ERC11PE PK100
EUR 13.5

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC11-10 PK100
EUR 12.15

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC11-10F PK5000
EUR 545.4

Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC11-10T PK1000
EUR 116.1

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC11-30 PK100
EUR 25.65

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC11-30F PK5000
EUR 1148.85

Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC11-30T PK1000
EUR 243

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK5000

CHSC9-35UBSF PK5000
EUR 1632.15

Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK1000

CHSC9-35UBST PK1000
EUR 344.25

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC20-2 PK100
EUR 59.4

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC20-2F PK5000
EUR 2718.9

Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC20-2T PK1000
EUR 573.75

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK100

CHSC9-20 PK100
EUR 20.25

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000

CHSC9-20F PK5000
EUR 907.2

Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK1000

CHSC9-20T PK1000
EUR 191.7

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK100

CHSC9-30 PK100
EUR 21.6

Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000

CHSC9-30F PK5000
EUR 967.95

Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK1000

CHSC9-30T PK1000
EUR 203.85

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000

CHSC9-40F PK5000
EUR 1451.25

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK1000

CHSC9-40T PK1000
EUR 306.45

Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC20-1 PK100
EUR 48.6

Silver headspace crimp cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC20-1F PK5000
EUR 2235.6

Silver crimp headspace cap (20mm) (Aluminium: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC20-1T PK1000
EUR 472.5

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK100

CHSC9-10 PK100
EUR 17.55

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000

CHSC9-10F PK5000
EUR 787.05

Blue screw cap (9mm) (PP: red natural rubber/clear TEF septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK1000

CHSC9-10T PK1000
EUR 166.05

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC20-1M PK100
EUR 74.25

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC20-1MF PK5000
EUR 3383.1

Magnetic gold crimp headspace cap (20mm) (Steel: grey butyl/grey PTFE molded septa; 3.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC20-1MT PK1000
EUR 714.15

Kimble Water analysis vial 40ml KG-33 clear glass with attached open top polypropylene Caps PTFE-faced silicone septa - PK72

VIA2518 PK72
EUR 288.9

Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) - preslit; with AVCS technology ; Level 1 Everyday Analysis - PK100

CHSC9-40 PK100
EUR 32.4

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK5000

CHCC20-2MF PK5000
EUR 5135.4

AgileSpec? UV-Visible Spectrophotometer, Split Beam (internal double beam), 190-1100nm, analysis software and automatic 6-cell holder included

ASP-1100 each
EUR 8456.4

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK100

CHCC20-2M PK100
EUR 112.05

Magnetic gold crimp headspace cap (20mm) (Steel: blue transparent silicone/clear PTFE soft septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK1000

CHCC20-2MT PK1000
EUR 1081.35

AgileSpec™ UV-Visible Spectrophotometer, Split Beam (internal double beam), 190-1100nm, analysis software and automatic 6-cell holder included

ASP 1100
EUR 6046.81

SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

M-PCR-378 500 µl
EUR 72
Description: SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service

M-PCR-379 500 µl
EUR 72
Description: EvaGreen® Fluorescent DNA StainDNA Intercalating dye for real-time qPCR fluorescence analysis including HRM** shipped ice packs - must be shipped via overnight service

Dry Immunofluorescence Analyzer (DIA)

DIA00101 1 set
EUR 3003.6
Description: Quantitative test strip fluorescence analyzer. Instrument rental is available at $300/15 days plus shipping & handling.

Fluorescence Immunoassay Analyzer

AFR-100/100S 1 kit Ask for price

Fluorescence Immunoassay Analyzer

AFR-200/200S 1 kit Ask for price

Fluorescence Immunoassay Analyzer

AFR-300/300S 1 kit Ask for price

Fluorescence Immunoassay Analyzer

AFR-302 1 kit Ask for price

Dry-type Fluorescent Immunoassay Analyzer

AFR-600 1 kit Ask for price

Up-converting Phosphor immunoassay analyzer

abx095263-1Machine 1 Machine
EUR 7326

16-Channel Fluorescence Immunoassay Analyzer

AFR-800 1 kit Ask for price

Automatic Chemiluminescence Immunoassay Analyzer

ACIR-100 1 kit Ask for price

Automatic Chemiluminescence Immunoassay Analyzer

ACIR-200 1 kit Ask for price

Moisture Analyser - 120g . 1mg - EACH

MOI1002 EACH
EUR 6012.9

MB27 Basic Moisture Analyser - EACH

BAL5550 EACH
EUR 2419.2

Infrared Moisture Analyser - EACH

MOI3050 EACH
EUR 3213

Analyser slider w rotating mount - EACH

MIC1584 EACH
EUR 188.01

HiQTM Immuno Block

I3211-010 100ml
EUR 160.8

HiQTM Immuno Block

I3211-050 500ml
EUR 319.2

Sartorius MA37-1 Moisture Analyser - EACH

BAL1150 EACH
EUR 5576.85

Purite Analyst 40/80/160 Annual Consumable Pack - EACH

WAT1090 EACH
EUR 1113.75
This prevents potential protein loss which will happen throughout centrifugation. As well as, this 2nd step concurrently shears the genomic DNA, making a further step for DNA fragmentation pointless.•The generated samples are appropriate for high-quality quantitative immunoblotting. With our RPE methodology we efficiently quantified the phosphorylated types of protein kinase GCN2 and its substrate eIF2α. In actual fact, the western alerts have been stronger and with much less background, as in comparison with samples generated with a pre-existing methodology.

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