Assay Blocking Blog calprotectin elisa camp elisa iron assay Isotype ldh assay kit mobp antibody Monoclonal p24 elisa Pcr pge2 elisa plc gamma 2 Serum tbars assay kit tdgf1 triglyceride assay upp1
A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry
Nitrotyrosine formation is attributable to presence of reactive oxygen and nitrogen species. Nitration is a really selective course of resulting in particular modification of only some tyrosines in protein molecule. 2D electrophoresis and western blotting strategies coupled with mass spectrometry are frequent strategies utilized in evaluation of proteome.
Right here we describe protocol for evaluation of peroxynitrite-induced protein nitration in remoted mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies in opposition to nitrotyrosine. Optimistic spots are in contrast with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry strategies.
Detection of UVR8 Homodimers and Monomers by Immunoblotting Evaluation in Tomato
The UV RESISTANCE LOCUS 8 (UVR8) is a photoreceptor mediating photomorphogenic responses to UV-B. UVR8 exists as homodimer in crops and UV-B induces dissociation of dimeric UVR8 into monomers to provoke responses. The monomer/dimer standing of UVR8 is reversible and a dynamic photo-equilibrium is established in crops in keeping with the ambient gentle circumstances.
Right here we describe a way to detect UVR8 homodimer and monomer by immunoblotting methodology from tomato (Solanum lycopersicum) crops. The characteristic of this methodology is that protein samples usually are not boiled previous to loading on an SDS-PAGE gel, which permits the detection of UVR8 homodimer and monomers concurrently with a single antibody.
Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative evaluation of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes
The Trichinella genus poses an attention-grabbing puzzle for researchers, having diverged very early within the evolution of the nematodes. The Trichinella spiralis proteome is a cosmopolitan and well-studied mannequin of Trichinella; nevertheless, Trichinella britovi additionally circulates within the sylvatic setting and each species infect people, ensuing within the improvement of trichinellosis. Few experiments have examined the proteins belonging to the T. britovi proteome.
The intention of the current research was to match the protein expression profiles of crude extracts of T. spiralis and T. britovi muscle larvae utilizing a highly-sensitive two-dimensional differential in-gel electrophoresis (2D DIGE) method coupled with 2DE immunoblotting. Chosen immunoreactive protein spots have been then recognized by liquid chromatography coupled with mass spectrometry evaluation (LC-MS/MS), and their operate in Trichinella and the host-parasite interplay was decided by gene ontology evaluation.
Spots frequent to each T. spiralis and T. britovi, spots with completely different expressions between the 2 and spots particular to every species have been labelled with completely different cyanine dyes. In whole, 196 protein spots have been present in each proteomes; of those 165 have been frequent, 23 expressed completely in T. spiralis and eight in T. britovi.
A comparative evaluation of quantity ratio values with Melanie software program confirmed that among the many frequent spots, 9 demonstrated greater expression in T. spiralis, and 17 in T. britovi. LC-MS/MS evaluation of 11 chosen spots recognized 41 proteins with potential antigenic traits: 26 have been particular for T. spiralis, six for T. britovi, and eight have been present in each proteomes.
Gene Ontology evaluation confirmed that the recognized T. spiralis proteins possess hydrolytic endopeptidase, endonuclease and transferase actions. Equally, many of the T. britovi proteins possess catalytic actions, akin to lyase, hydrolase, isomerase and peptidase exercise. The utilized 2D DIGE method visualized Trichinella spp. protein spots with completely different molecular weights or isoelectric level values, in addition to these with completely different expression ranges.
The recognized immunoreactive proteins take part in a number of processes related to host muscle cell invasion and larval adaptation to the host setting. Their reactivity with the host immune system makes them potential candidates for the event of a novel trichinellosis diagnostic check or vaccine in opposition to helminthiasis attributable to T. spiralis or T. britovi.’
Immunoblotting Evaluation of Intracellular PD-L1 Ranges in Interferon-γ-Handled Ovarian Most cancers Cells Stably Transfected with Bcl3 shRNA.
Expression of the immune checkpoint programmed loss of life ligand 1 (PD-L1, CD274) is elevated in lots of sorts of most cancers, together with ovarian most cancers (OC), however the mechanisms that regulate the PD-L1 expression usually are not totally understood. Along with binding to PD-1 on T cells, thus inhibiting T cell-mediated antitumor responses, PD-L1 has additionally tumor-intrinsic results that embody elevated most cancers cell survival and proliferation, and that is perhaps partially mediated by the intracellular PD-L1.
On this chapter, we describe a protocol for the evaluation of the intracellular PD-L1 protein ranges in OC cells by immunoblotting. Our outcomes present that interferon-γ (IFNγ) induces the intracellular ranges of PD-L1 and the proto-oncogene Bcl3 in OC cells.
Nevertheless, the PD-L1 expression is considerably decreased in OC cells stably transfected with Bcl3 shRNA, demonstrating that the IFNγ-induced PD-L1 expression in OC cells is mediated by Bcl3. These information determine the IFNγ-Bcl3-PD-L1 axis as a novel therapeutic goal in OC, and counsel that concentrating on Bcl3 might present a novel technique to manage the PD-L1 expression, and particularly the tumor-intrinsic PD-L1 results mediated by the intracellular PD-L1 in OC cells.
A Fast Extraction Technique for mammalian cell cultures, appropriate for quantitative immunoblotting evaluation of proteins, together with phosphorylated GCN2 and eIF2α.
Many research require the detection and relative quantitation of proteins from cell tradition samples utilizing immunoblotting. Limiting elements are the price of protease inhibitors, the time required to interrupt cells and generate samples, in addition to the excessive threat of protein loss throughout cell breakage procedures. As well as, a typical downside is the viscosity of lysed samples because of the launched genomic DNA.
As a consequence, the DNA must be damaged down previous to denaturing polyacrylamide protein gel electrophoresis (SDS-PAGE), e.g. by passing the pattern by way of a syringe gauge needle, sonication, or DNase therapy.
In a quest to discover a less expensive, quick, and but sturdy process, we discovered that cell lysis, protein denaturation, and DNA fragmentation could be finished in solely two steps: harvesting adopted by a easy non-laborious 2nd step. Equally to many pre-existing cell breakage procedures, in our Fast Protein Extraction (RPE) methodology, proteins liberated from cells are instantly uncovered to a denaturing setting.
Nevertheless, benefits of our methodology are: •No breaking buffer is required, as an alternative proteins are liberated immediately into the denaturing protein loading buffer used for SDS-PAGE. Consequently, our RPE methodology doesn’t require any costly inhibitors.•The RPE methodology doesn’t contain post-lysis centrifugation steps; as an alternative all cell materials is dissolved in the course of the 2nd step, the mixing-heat-treatment step which is new to this methodology.
HT-MSI+ Analysis Kit |
|||
| PCR-704 | Jena Bioscience GmbH | 100reactions | EUR 1125.53 |
Cell Cycle Analysis Kit |
|||
| CCA100 | Ecotech Biotechnology | 100 rxn | EUR 184.8 |
|
Description: NutriCulture Cell Cycle Analysis Kit detects alterations in cell cycle by measuring DNA content. NutriCulture Cell Cycle Analysis Kit is intended to be used to detect the DNA content (cell cycle) of cultured suspension or adherent cells. |
|||
Cell Cycle Analysis Kit |
|||
| CCA20 | Ecotech Biotechnology | 20 rxn | EUR 46.2 |
|
Description: NutriCulture Cell Cycle Analysis Kit detects alterations in cell cycle by measuring DNA content. NutriCulture Cell Cycle Analysis Kit is intended to be used to detect the DNA content (cell cycle) of cultured suspension or adherent cells. |
|||
Cell Cycle Analysis Kit |
|||
| CCA50 | Ecotech Biotechnology | 50 rxn | EUR 92.4 |
|
Description: NutriCulture Cell Cycle Analysis Kit detects alterations in cell cycle by measuring DNA content. NutriCulture Cell Cycle Analysis Kit is intended to be used to detect the DNA content (cell cycle) of cultured suspension or adherent cells. |
|||
Acetone, 99.8%, for analysis |
|||
| GK3913 | Glentham Life Sciences | 500ml | EUR 72.46 |
Acetone, 99.8%, for analysis |
|||
| GK3913-1 | Glentham Life Sciences | 1 | EUR 38.8 |
Acetone, 99.8%, for analysis |
|||
| GK3913-1L | Glentham Life Sciences | 1 l | EUR 82.8 |
Acetone, 99.8%, for analysis |
|||
| GK3913-2500 | Glentham Life Sciences | 2500 | EUR 79.1 |
Acetone, 99.8%, for analysis |
|||
| GK3913-2500ML | Glentham Life Sciences | 2500 ml | EUR 132 |
Acetone, 99.8%, for analysis |
|||
| GK3913-500 | Glentham Life Sciences | 500 | EUR 24.6 |
Acetone, 99.8%, for analysis |
|||
| GK3913-500ML | Glentham Life Sciences | 500 ml | EUR 66 |
Hydroxyproline analysis 11-20 samples |
|||
| 80062 | Chondrex | Custom service | EUR 167.88 |
|
Description: Hydroxyproline analysis 11-20 samples |
|||
Hydroxyproline analysis 31-40 samples |
|||
| 80064 | Chondrex | Custom service | EUR 159.18 |
|
Description: Hydroxyproline analysis 31-40 samples |
|||
Collagen analysis, <10 samples |
|||
| 80031 | Chondrex | Custom service | EUR 174.84 |
|
Description: Collagen analysis |
|||
Collagen analysis, >30 samples |
|||
| 80033 | Chondrex | Custom service | EUR 162.66 |
|
Description: Collagen analysis |
|||
Rtg Rapid Analysis Kit - EACH |
|||
| 27950201 | Scientific Laboratory Supplies | EACH | EUR 1482.3 |
Rtg Rapd Analysis Beads - EACH |
|||
| 27950001 | Scientific Laboratory Supplies | EACH | EUR 1074.6 |
DI PAK FOR NP ANALYSIS - EACH |
|||
| CPDI0NJM1 | Scientific Laboratory Supplies | EACH | EUR 384.83 |
Nutrient analysis photometer - EACH |
|||
| SPE2110 | Scientific Laboratory Supplies | EACH | EUR 958.5 |
Hydroxyproline analysis <10 samples |
|||
| 80061 | Chondrex | Custom service | EUR 171.36 |
|
Description: Hydroxyproline analysis <10 samples |
|||
DNA Ploidy Analysis Staining Kit |
|||
| K1439-1 | Biovision | each | EUR 705.6 |
DNA Ploidy Analysis Staining Kit |
|||
| K1439-5 | Biovision | each | EUR 2402.4 |
EZCellTM Cell Cycle Analysis Kit |
|||
| K920-100 | Biovision | each | EUR 451.2 |
Peroxide Block for Image Analysis |
|||
| ADA015 | ScyTek Laboratories | 15 ml | EUR 16.72 |
Peroxide Block for Image Analysis |
|||
| ADA500 | ScyTek Laboratories | 500 ml | EUR 61.72 |
Peroxide Block for Image Analysis |
|||
| ADA999 | ScyTek Laboratories | 1000 ml | EUR 86.15 |
ANA 9 Line Immunoblot 08 ELISA kit |
|||
| 55R-ORG710/08 | Fitzgerald | 8 Tests | EUR 165 |
|
Description: ELISA kit for the detection of ANA 9 Line Immunoblot 08 in the research laboratory |
|||
ANA 9 Line Immunoblot 16 ELISA kit |
|||
| 55R-ORG710/16 | Fitzgerald | 16 Tests | EUR 300 |
|
Description: ELISA kit for the detection of ANA 9 Line Immunoblot 16 in the research laboratory |
|||
Antibody analysis - human >30 samples |
|||
| 8005-3 | Chondrex | Custom service | EUR 136.56 |
|
Description: Antibody analysis - human >30 samples |
|||
Antibody analysis - human <10 samples |
|||
| 80051 | Chondrex | Custom service | EUR 147 |
|
Description: Antibody analysis - human <10 samples |
|||
XELEX DNA CORE Kit (Analysis Unit) |
|||
| E3652-01 | EURx | 25analysis | EUR 119.9 |
Sodium dodecyl sulfate, for analysis |
|||
| GX5289 | Glentham Life Sciences | 25g | EUR 94 |
Sodium dodecyl sulfate, for analysis |
|||
| GX5289-100 | Glentham Life Sciences | 100 | EUR 102.7 |
Sodium dodecyl sulfate, for analysis |
|||
| GX5289-25 | Glentham Life Sciences | 25 | EUR 34.8 |
PKU Serum LC-MS/MS Analysis Kit |
|||
| ZV-3003-0200-10 | Zivak Technologies | 200 Tests | Ask for price |
Spatular Analysis type 500mm SS - EACH |
|||
| DD001810 | Scientific Laboratory Supplies | EACH | EUR 37.8 |
Exosome size distribution analysis (NTA) |
|||
| S108 | 101Bio | - | EUR 600 |
Antibody analysis - human 10-30 samples |
|||
| 8005-2 | Chondrex | Custom service | EUR 140.04 |
|
Description: Antibody analysis - human 10-30 samples |
|||
TraceDrawer Analysis Package with Laptop |
|||
| NITDS-P | Westburg | each | EUR 5722.5 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
| GK0126 | Glentham Life Sciences | 1l | EUR 159.23 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
| GK0126-1 | Glentham Life Sciences | 1 | EUR 83.1 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
| GK0126-1L | Glentham Life Sciences | 1 l | EUR 136.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
| GK0126-2500 | Glentham Life Sciences | 2500 | EUR 174 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
| GK0126-2500ML | Glentham Life Sciences | 2500 ml | EUR 246 |
Organic Acids UrineLC-MS/MS Analysis Kit |
|||
| ZV-3004-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
NeurotransmittersUrine LC-MS/MS Analysis Kit |
|||
| ZV-3033-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
Catecholamines Urine LC-MS/MS Analysis Kit |
|||
| ZV-3031-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
Blue Feulgen DNA Ploidy Analysis Staining Kit |
|||
| DPK500 | ScyTek Laboratories | 1 kit(s) | EUR 245.58 |
Biogenic Amins Urine LC-MS/MS Analysis Kit |
|||
| ZV-3065-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
Kjeldahl Analysis Weighing Boat Grade 609 - PK100 |
|||
| WHA10313032 | Scientific Laboratory Supplies | EACH | EUR 147.15 |
2,3,5-Triphenyltetrazolium chloride, 99%, for analysis |
|||
| GE2454 | Glentham Life Sciences | 5g | EUR 103.51 |
2,3,5-Triphenyltetrazolium chloride, 99%, for analysis |
|||
| GE2454-25 | Glentham Life Sciences | 25 | EUR 113.1 |
2,3,5-Triphenyltetrazolium chloride, 99%, for analysis |
|||
| GE2454-5 | Glentham Life Sciences | 5 | EUR 37.7 |
PKU Serum LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3003-KK-10 | Zivak Technologies | each | Ask for price |
Antibody analysis - rat, mouse, or human <10 samples |
|||
| 80021 | Chondrex | Custom service | EUR 138.3 |
|
Description: Antibody analysis - rat |
|||
Antibody analysis - rat, mouse, or human >30 samples |
|||
| 80023 | Chondrex | Custom service | EUR 127.86 |
|
Description: Antibody analysis - rat |
|||
GMDM Urine LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3060-KK-20 | Zivak Technologies | 1 | Ask for price |
VMA HVA 5-HIAA Urine LC-MS/MS Analysis Kit |
|||
| ZV-3030-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
PKU Dried Blood Spot DBS LC-MS/MS Analysis Kit |
|||
| ZV-3003-0200-15 | Zivak Technologies | 200 Tests | Ask for price |
C-Series Mouse Inflammation Array Analysis Software |
|||
| S02-AAM-INF-1 | Neuromics | CD | EUR 325.2 |
Antibody analysis - rat, mouse, or human 10-30 samples |
|||
| 80022 | Chondrex | Custom service | EUR 133.08 |
|
Description: Antibody analysis - rat |
|||
Antibody analysis - chick, bovine, or porcine <10 samples |
|||
| 80011 | Chondrex | Custom service | EUR 129.6 |
|
Description: Antibody analysis - chick |
|||
Antibody analysis - chick, bovine, or porcine >30 samples |
|||
| 80013 | Chondrex | Custom service | EUR 117.42 |
|
Description: Antibody analysis - chick |
|||
Exo-Flow 2.0 CD9 Analysis Kit (for Serum or Plasma) |
|||
| EXOFLOW2-105A-SP | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma) |
|||
| EXOFLOW2-100A-SP | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD81 Analysis Kit (for Serum or Plasma) |
|||
| EXOFLOW2-110A-SP | SBI | 10 rxn | EUR 705.6 |
Amino Acid Biological Fluids LC-MS/MS Analysis Kit |
|||
| ZV-3002-0200-10 | Zivak Technologies | 200 Tests | Ask for price |
On site set up for 1 Zivak LC-MS/MS analysis kit |
|||
| ZE-1000-0001-03 | Zivak Technologies | each | Ask for price |
Rubella Virus IgG Immunoblot |
|||
| GWB-521231 | GenWay Biotech | 1x96 Assays | Ask for price |
ROCK Activity Immunoblot Kit |
|||
| MBS168582-20Assays | MyBiosource | 20Assays | EUR 710 |
ROCK Activity Immunoblot Kit |
|||
| MBS168582-5x20Assays | MyBiosource | 5x20Assays | EUR 3275 |
OxiSelect MDA Immunoblot Kit |
|||
| STA-331 | Cell Biolabs | 10 blots | EUR 664.8 |
|
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Immunoblot Kit provides a specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
|||
ROCK Activity Immunoblot Kit |
|||
| STA-415 | Cell Biolabs | 20 assays | EUR 436 |
Urine Analysis Tube 12ml 16 x 100mm polystyrene - PK1600 |
|||
| G160101 | Scientific Laboratory Supplies | PK1600 | EUR 93.15 |
Organic Acid Urine LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3004-KK-20 | Zivak Technologies | 1 | Ask for price |
Antibody analysis - chick, bovine, or porcine 10-30 samples |
|||
| 80012 | Chondrex | Custom service | EUR 122.64 |
|
Description: Antibody analysis - chick |
|||
HR Pooled Cell analysis: 1000ug DR-HRGFP, 10000ug pSce1, 25ug e-GFP, Puromycin |
|||
| DR31000 | TopoGen | 1 | EUR 3423.6 |
Exo-Flow 2.0 CD9 Analysis Kit (for Tissue Culture Media) |
|||
| EXOFLOW2-205A-TC | SBI | 10 rxn | EUR 705.6 |
Vitamin ADE SerumLCMS-MS APCI Analysis Kit User Manuel |
|||
| ZV-3059-KK-10 | Zivak Technologies | 1 | Ask for price |
Exo-Flow 2.0 CD63 Analysis Kit (for Tissue Culture Media) |
|||
| EXOFLOW2-200A-TC | SBI | 10 rxn | EUR 705.6 |
Exo-Flow 2.0 CD81 Analysis Kit (for Tissue Culture Media) |
|||
| EXOFLOW2-210A-TC | SBI | 10 rxn | EUR 705.6 |
Carnitine/Acylcarnitines Dried BloodLC-MS/MS Analysis Kit |
|||
| ZV-3051-0200-15 | Zivak Technologies | 200 Tests | Ask for price |
84us Conductivity Solution with certificate of analysis - EACH |
|||
| HI-6033 | Scientific Laboratory Supplies | EACH | EUR 43.2 |
PKU Dried Blood Spots LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3003-KK-15 | Zivak Technologies | 1 | Ask for price |
Neonatal Screening Dried Blood Spots LCMS-MS Analysis Kit |
|||
| ZV-3017-0500-15 | Zivak Technologies | 500 Tests | Ask for price |
25-HydroxyvitaminD2-D3 SerumLC-MS/MS ESI Analysis Kit |
|||
| ZV-3045-0200-10 | Zivak Technologies | 200 Tests | Ask for price |
HR Pooled Cell analysis: 500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP, Puromycin |
|||
| DR3500 | TopoGen | 1 | EUR 3174 |
Biogenic Amins in Urine LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3065-KK-20 | Zivak Technologies | 1 | Ask for price |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK100 |
|||
| CHBS-20 | Scientific Laboratory Supplies | PK100 | EUR 17.55 |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK5000 |
|||
| CHBS-20F | Scientific Laboratory Supplies | PK5000 | EUR 787.05 |
Stopper (20mm) (grey bromobutyl) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHBS-20T | Scientific Laboratory Supplies | PK1000 | EUR 166.05 |
Neurotransmitters in Urine LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3033-KK-20 | Zivak Technologies | 1 | Ask for price |
Amino Acid Biological Fluids LC-MS/MS Analysis Kit User manual |
|||
| ZV-3002-KK-10 | Zivak Technologies | 1 | Ask for price |
Exo-FACS ready to use kit for analysis of human SERUM exosomes |
|||
| HBM-FACS-PES | HansaBioMed | KIT | EUR 272.16 |
Exo-FACS ready to use kit for analysis of human URINE exosomes |
|||
| HBM-FACS-PEU | HansaBioMed | KIT | EUR 347.76 |
1x20 SACHET 25ml pH 4,01 ± 0,02 / 25°C (red), with Certificate of Analysis |
|||
| 10002013 | Phoenix instrument | each | EUR 29 |
OxiSelect™ Protein Carbonyl Immunoblot Kit |
|||
| STA-308 | Cell Biolabs | 10 blots | EUR 340 |
|
Description: Excepted Quantities |
|||
Checkpoint Kinase Activity Immunoblot Kit |
|||
| STA-413 | Cell Biolabs | 20 assays | EUR 651.6 |
|
Description: Cell Biolabs? Checkpoint Kinase Activity Immunoblot Kit utilizes recombinant Cdc25C as checkpoint kinase substrate. After incubating the substrate with checkpoint kinase samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated Cdc25C is detected by western blot analysis using an anti-phospho-Cdc25C (Ser216). |
|||
Exo-FACS ready to use kit for analysis of human PLASMA exosomes |
|||
| HBM-FACS-PEP | HansaBioMed | KIT | EUR 272.16 |
Gut Microbiota Dysbiosis Marker Urine (GMDM) LC-MS/MS Analysis Kit |
|||
| ZV-3060-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
1x20 SACHET 25ml EC 1413 µS/cm ± 1% / 25°C, with Certificate of Analysis |
|||
| 10002053 | Phoenix instrument | each | EUR 29 |
1x20 SACHET 25ml EC 12880 µS/cm ± 1% / 25°C, with Certificate of Analysis |
|||
| 10002063 | Phoenix instrument | each | EUR 29 |
1x20 SACHET 25ml pH 7,00 ± 0,02 / 25°C (green), with Certificate of Analysis |
|||
| 10002023 | Phoenix instrument | each | EUR 29 |
Customized Organic Acids Panel-2 in Urine LC-MS/MS Analysis Kit |
|||
| ZV-3066-0200-20 | Zivak Technologies | 200 Tests | Ask for price |
1x20 SACHET 25ml pH 10,01 ± 0,02 / 25°C (yellow), with Certificate of Analysis |
|||
| 10002043 | Phoenix instrument | each | EUR 29 |
Nitrotyrosine, Immunoblot Positive Control (nTyr) |
|||
| MBS657779-0065mg | MyBiosource | 0.065mg | EUR 675 |
Nitrotyrosine, Immunoblot Positive Control (nTyr) |
|||
| MBS657779-5x0065mg | MyBiosource | 5x0.065mg | EUR 2880 |
OxiSelect Nitrotyrosine Protein Immunoblot Kit |
|||
| STA-303 | Cell Biolabs | 10 blots | EUR 490.8 |
|
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine Immunoblot Kit provides a simple method to measure the formation of 3-nitrotyrosine in proteins by Western blot detection. |
|||
OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size |
|||
| STA-308-T | Cell Biolabs | 4 blots | EUR 316.8 |
|
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. |
|||
Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
|||
| DD2R | Detroit R&D | 1 Kit | EUR 550 |
Neonatal Screening Dried Blood Spots LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3017-KK-15 | Zivak Technologies | 1 | Ask for price |
Oxidized Protein Immunoblot Control (Carbonyl-BSA) |
|||
| MBS168896-001mg | MyBiosource | 0.01mg | EUR 285 |
Oxidized Protein Immunoblot Control (Carbonyl-BSA) |
|||
| MBS168896-5x001mg | MyBiosource | 5x0.01mg | EUR 1120 |
Oxidized Protein Immunoblot Control (Carbonyl-BSA) |
|||
| STA-309 | Cell Biolabs | 10 ?g | EUR 144 |
Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
|||
| DD2H | Detroit R&D | 1 Kit | EUR 550 |
Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
|||
| DD2M | Detroit R&D | 1 Kit | EUR 550 |
HR Pooled Cell analysis: 50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP, Puromycin |
|||
| DR3050 | TopoGen | 1 | EUR 2175.6 |
Exo-FACS ready to use kit for analysis of exosomes markers from cell media |
|||
| HBM-FACS-C | HansaBioMed | KIT | EUR 347.76 |
Carnitine/Acylcarnitine Dried Blood Spots LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3051-KK-15 | Zivak Technologies | 1 | Ask for price |
Exo-FACS ready to use kit for analysis of exosomes markers from MSC exosomes |
|||
| HBM-FACS-MSC | HansaBioMed | KIT | EUR 393.12 |
Mikrogen Diagnostik recomBlot Rubella IgG Immunoblot Test Kit |
|||
| 4902 | Genovis AB | 20 Tests | EUR 660 |
OxiSelect N-epsilon-(Carboxymethyl) Lysine (CML) Immunoblot Kit |
|||
| STA-313 | Cell Biolabs | 10 blots | EUR 637.2 |
|
Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Immunoblot kit specifically detects CML formation in protein samples. |
|||
Customized Organic Acids Panel-2 in Urine LC-MS/MS Analysis Kit User Manual |
|||
| ZV-3066-02KK-20 | Zivak Technologies | 1 | Ask for price |
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence* |
|||
| 15291 | AAT Bioquest | 100 Tests | EUR 570 |
|
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
|||
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence* |
|||
| 15291-100Tests | AAT Bioquest | 100 Tests | EUR 558 |
|
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
|||
qPCR Mastermix EVA (2X) (SafeLine: EvaGreen, dTTP) for HRM Analysis packed in 1,25 ml vials |
|||
| S150 | GeneOn | 100 rcs (2,5 ml) | EUR 155 |
qPCR Mastermix EVA (2X) (SafeLine: EvaGreen, dTTP) for HRM Analysis packed in 1,25 ml vials |
|||
| S150L | GeneOn | 500 rcs (12,5 ml) | EUR 1250 |
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence* |
|||
| 15296 | AAT Bioquest | 100 Tests | EUR 570 |
|
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
|||
Cell Meterâ„¢ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence* |
|||
| 15296-100Tests | AAT Bioquest | 100 Tests | EUR 558 |
|
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. |
|||
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK100 |
|||
| CHSV9-10P | Scientific Laboratory Supplies | PK100 | EUR 12.15 |
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSV9-10PF | Scientific Laboratory Supplies | PK5000 | EUR 545.4 |
2mL clear screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK1000 |
|||
| CHSV9-10PT | Scientific Laboratory Supplies | PK1000 | EUR 116.1 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK100 |
|||
| CHSV9-20P | Scientific Laboratory Supplies | PK100 | EUR 16.2 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSV9-20PF | Scientific Laboratory Supplies | PK5000 | EUR 726.3 |
2mL amber screw glass vial (9mm short thread) with write-on patch ; Level 1 Everyday Analysis - PK1000 |
|||
| CHSV9-20PT | Scientific Laboratory Supplies | PK1000 | EUR 153.9 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis |
|||
| CHCV20-14F | Scientific Laboratory Supplies | PK5000 | EUR 1226.64 |
2mL clear crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK100 |
|||
| CHCV11-10P | Scientific Laboratory Supplies | PK100 | EUR 13.5 |
2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK5000 |
|||
| CHCV11-10PF | Scientific Laboratory Supplies | PK5000 | EUR 606.15 |
2mL clear cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCV11-10PT | Scientific Laboratory Supplies | PK1000 | EUR 128.25 |
2mL amber crimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK100 |
|||
| CHCV11-20P | Scientific Laboratory Supplies | PK100 | EUR 17.55 |
2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK5000 |
|||
| CHCV11-20PF | Scientific Laboratory Supplies | PK5000 | EUR 787.05 |
2mL amber cimp glass vial (11mm thread: wide opening) with write-on patch ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCV11-20PT | Scientific Laboratory Supplies | PK1000 | EUR 166.05 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK100 |
|||
| CHSC9-35UB | Scientific Laboratory Supplies | PK100 | EUR 29.7 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSC9-35UBF | Scientific Laboratory Supplies | PK5000 | EUR 1329.75 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHSC9-35UBT | Scientific Laboratory Supplies | PK1000 | EUR 280.8 |
HeLa Cell RepHR Pooled Cell analysis: 250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP, Puromycinorters to detect DNA Hypomethylating Effectors |
|||
| DR3250 | TopoGen | 1 | EUR 2550 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis - PK100 |
|||
| CHCV20-14 | Scientific Laboratory Supplies | PK100 | EUR 33.75 |
20mL clear crimp glass vial (20mm) Headspace (square edge round bottom) (22.5 x 75.5mm) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCV20-14T | Scientific Laboratory Supplies | PK1000 | EUR 319.95 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK100 |
|||
| CHSC9-35UBS | Scientific Laboratory Supplies | PK100 | EUR 36.45 |
CAP SNAP 11MM BLUE POLYETHYLENE INTEGRAL PE SEPTUM THINNED PENETRATION AREA 100/PACK ; Level 1 Everyday Analysis - PK100 |
|||
| 6ERC11PE | Scientific Laboratory Supplies | PK100 | EUR 13.5 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK100 |
|||
| CHCC11-10 | Scientific Laboratory Supplies | PK100 | EUR 12.15 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK5000 |
|||
| CHCC11-10F | Scientific Laboratory Supplies | PK5000 | EUR 545.4 |
Silver crimp cap (11mm) (Aluminium: natural rubber/clear TEF septa; 60 Shore: 1.0mm thickness) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCC11-10T | Scientific Laboratory Supplies | PK1000 | EUR 116.1 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK100 |
|||
| CHCC11-30 | Scientific Laboratory Supplies | PK100 | EUR 25.65 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK5000 |
|||
| CHCC11-30F | Scientific Laboratory Supplies | PK5000 | EUR 1148.85 |
Silver crimp cap (11mm) (Aluminium: white silicone/red PTFE septa; 45 Shore: 1.3mm thickness) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCC11-30T | Scientific Laboratory Supplies | PK1000 | EUR 243 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSC9-35UBSF | Scientific Laboratory Supplies | PK5000 | EUR 1632.15 |
Bonded blue screw cap (9mm) (PP: white silicone/white PTFE septa: 1.0mm thickness) -preslit ; Level 1 Everyday Analysis - PK1000 |
|||
| CHSC9-35UBST | Scientific Laboratory Supplies | PK1000 | EUR 344.25 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK100 |
|||
| CHCC20-2 | Scientific Laboratory Supplies | PK100 | EUR 59.4 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK5000 |
|||
| CHCC20-2F | Scientific Laboratory Supplies | PK5000 | EUR 2718.9 |
Silver crimp headspace cap (20mm) (Aluminium: blue silicone/white PTFE septa: 3.0mm thickness) ; Level 1 Everyday Analysis - PK1000 |
|||
| CHCC20-2T | Scientific Laboratory Supplies | PK1000 | EUR 573.75 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK100 |
|||
| CHSC9-20 | Scientific Laboratory Supplies | PK100 | EUR 20.25 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSC9-20F | Scientific Laboratory Supplies | PK5000 | EUR 907.2 |
Blue screw cap (9mm) (PP: red silicone/ivory PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK1000 |
|||
| CHSC9-20T | Scientific Laboratory Supplies | PK1000 | EUR 191.7 |
Blue screw cap (9mm) (PP: white silicone/blue PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK100 |
|||
| CHSC9-30 | Scientific Laboratory Supplies | PK100 | EUR 21.6 |
Blue screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology ; Level 1 Everyday Analysis - PK5000 |
|||
| CHSC9-30F | Scientific Laboratory Supplies | PK5000 | EUR 967.95 |
This prevents potential protein loss which will happen throughout centrifugation. As well as, this 2nd step concurrently shears the genomic DNA, making a further step for DNA fragmentation pointless.•The generated samples are appropriate for high-quality quantitative immunoblotting. With our RPE methodology we efficiently quantified the phosphorylated types of protein kinase GCN2 and its substrate eIF2α. In actual fact, the western alerts have been stronger and with much less background, as in comparison with samples generated with a pre-existing methodology.
