immunoblotting analysis, A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry

Nitrotyrosine formation is attributable to presence of reactive oxygen and nitrogen species. Nitration is a really selective course of resulting in particular modification of only some tyrosines in protein molecule. 2D electrophoresis and western blotting strategies coupled with mass spectrometry are frequent strategies utilized in evaluation of proteome. Right here we describe protocol for evaluation…

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