Neurosteroidogenic Enzymes
Neurosteroidogenic Enzymes: CYP11A1 within the Central Nervous System
Neurosteroids, steroid hormones synthesized regionally in the nervous system, have necessary neuromodulatory and neuroprotective results within the central nervous system. Progress in neurosteroid analysis has led to the profitable translation of allopregnanolone into an permitted remedy for postpartum despair. Nevertheless, there may be inadequate proof to help the assumption that steroidogenesis is precisely the identical between the nervous system and the periphery.
This evaluation focuses on CYP11A1, the one enzyme at present recognized to catalyze the primary response in steroidogenesis to supply pregnenolone, the precursor to all different steroids. Though CYP11A1 mRNA has been present in mind of many mammals, the presence of CYP11A1 protein has been troublesome to detect, significantly in people. Right here, we spotlight the discrepancies within the present proof for CYP11A1 within the central nervous system and suggest new instructions for understanding neurosteroidogenesis, which can be essential for creating neurosteroid-based therapies for the longer term.
Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit |
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| GWB-892399 | GenWay Biotech | ELISA_Kits | Ask for price |
Beta-2-Microglobulin Enzyme Immunoassay Test Kit |
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| GWB-8C575E | GenWay Biotech | ELISA_Kits | Ask for price |
Cancer Antigen CA19-9 Enzyme Immunoassay Test Kit |
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| GWB-EED29A | GenWay Biotech | ELISA_Kits | Ask for price |
Carcinoembryonic Antigen (CEA) Enzyme Immunoassay Test Kit |
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| GWB-FB64BF | GenWay Biotech | ELISA_Kits | Ask for price |
Human Growth Hormone (HGH) Enzyme Immunoassay Test Kit |
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| GWB-F6BC01 | GenWay Biotech | ELISA_Kits | Ask for price |
Prostate Specific Antigen (PSA) Enzyme Immunoassay Test Kit |
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| GWB-5B3CF0 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-6B71D4-1U - Progesterone Enzyme Immunoassay Test Kit |
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| GWB-6B71D4-1U | Aviva Systems Biology | 96Wells | EUR 249 |
Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit |
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| GWB-65C26B | GenWay Biotech | ELISA_Kits | Ask for price |
Human Chorionic Gonadotropin (hCG) Enzyme immunoassay Test Kit |
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| GWB-E3238F | GenWay Biotech | ELISA_Kits | Ask for price |
Rat Prolactin Chemiluminescent Immunoassay Kit |
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| IRTPRLKTC | Innovative research | each | EUR 545 |
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Description: Rat Prolactin Chemiluminescent Immunoassay Kit |
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Breast Cancer Antigen CA15-3 Enzyme Immunoassay Test Kit |
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| GWB-150A4D | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-AFC61F-1U - Estradiol (E2) Enzyme Immunoassay Test Kit |
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| GWB-AFC61F-1U | Aviva Systems Biology | 96Wells | EUR 379 |
Follicle-Stimulating Hormone (FSH) Enzyme Immunoassay Test Kit |
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| GWB-44F874 | GenWay Biotech | ELISA_Kits | Ask for price |
Human Prolactin Chemiluminescent Immunoassay Kit |
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| IHUPRLKTC | Innovative research | each | EUR 545 |
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Description: Human Prolactin Chemiluminescent Immunoassay Kit |
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Mouse Prolactin Chemiluminescent Immunoassay Kit |
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| IMSPRLKTC | Innovative research | each | EUR 545 |
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Description: Mouse Prolactin Chemiluminescent Immunoassay Kit |
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GWB-5185FE-1U - HSV-2 IgG Enzyme Immunoassay Test Kit |
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| GWB-5185FE-1U | Aviva Systems Biology | 96wells | EUR 329 |
Troponin-I (Human Cardiac-Specific) Enzyme Immunoassay Test Kit |
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| GWB-83A61F | GenWay Biotech | ELISA_Kits | Ask for price |
High Sensitivity C-Reactive Protein (CRP) Enzyme Immunoassay Test Kit |
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| GWB-C97162 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-892399-96W - Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit |
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| GWB-892399-96W | Aviva Systems Biology | 96wells | EUR 329 |
Rapid Visual Human Chorionic Gonadotropin (hCG) Enzyme Immunoassay Test Kit |
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| GWB-DAB550 | GenWay Biotech | ELISA_Kits | Ask for price |
ULTRASENSITIVE THYROID STIMULATING HORMONE (U-TSH) ENZYME IMMUNOASSAY TEST KIT |
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| GWB-8BFFB0 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-DAB550-1U - Rapid Visual Human Chorionic Gonadotropin (hCG) Enzyme Immunoassay Test Kit |
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| GWB-DAB550-1U | Aviva Systems Biology | 96Wells | EUR 219 |
Aldosterone Enzyme Immunoassay Kit |
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| DEIABL242 | Creative Diagnostics | 96T | EUR 1026 |
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Description: The Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total aldosterone in extracted serum or plasma and fecal samples. |
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Sunlong Medical™ Mouse AXL Enzyme Immunoassay Kit |
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| EL0123Mo-48T | Sunlong | 48T | EUR 398 |
Sunlong Medical™ Mouse AXL Enzyme Immunoassay Kit |
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| EL0123Mo-96T | Sunlong | 96T | EUR 569 |
Free Thyroxine (fT4) Enzyme Immunoassy Test Kit |
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| GWB-9857BC | GenWay Biotech | ELISA_Kits | Ask for price |
Human Estrone-3-Glucuronide (E1G)Enzyme Immunoassay ELISA Kit |
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| QY-E05423 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit |
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| IHUIDEKTC | Innovative research | each | EUR 545 |
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Description: Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit |
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Alpha-Fetoprotein (AFP) Enzyme Immunoassy Test Kit |
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| GWB-C3BE7B | GenWay Biotech | ELISA_Kits | Ask for price |
Free Triiodothyronine (fT3) Enzyme Immunoassy Test Kit |
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| GWB-AA1FE4 | GenWay Biotech | ELISA_Kits | Ask for price |
Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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| IHUACEKTC | Innovative research | each | EUR 545 |
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Description: Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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| IMSACEKTC | Innovative research | each | EUR 545 |
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Description: Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit |
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| IHUACE2KTC | Innovative research | each | EUR 545 |
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Description: Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit |
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Canine Cortisol Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG017 | EnoGene | 10 Tests/Kit | EUR 63 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Progesterone (cProg) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG016 | EnoGene | 10 Tests/Kit | EUR 63 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Pancrea Lipase (cPL) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG014 | EnoGene | 10 Tests/Kit | EUR 70 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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D- Dimer Test Kit (Fluorescence Lateral Flow Immunoassay) , For Canine/Feline |
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| HG020 | EnoGene | 10 Tests/Kit | EUR 70 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine C-reactive Protein (cCRP) Test kit (Fluorescence Lateral Flow Immunoassay) |
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| HG012 | EnoGene | 10 Tests/Kit | EUR 63 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Total Thyroxine (T4) EnzymeE Immunoassy Test Kit |
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| GWB-F63F68 | GenWay Biotech | ELISA_Kits | Ask for price |
Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Colloidal gold immunoassay) |
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| CP04010 | UnScience | 25T/kit | Ask for price |
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Description: The Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Serum/Plasma/Whole blood) is a qualitative membrane-based immunoassay for the detection of SARS-CoV-2 neutralizing antibodies in serum, plasma and whole blood. The sample is dropped into the sample well, and chromatography is performed under the capillary effect. The SARS-CoV-2 neutralizing antibodies in the sample combined with the colloidal gold-labeled SARS-CoV-2 spike protein(SP), then spread to the test area. It is captured by coated SP subunit RBDNTD-CTD, to form a complex and gather in the test area (T line). The quality control area is coated with mouse anti-chicken IgY, and the colloidal goldlabeled chicken IgY is captureed to form a complex and aggregate in hte quality control area (C line). If the C line does not show color, it indicates that the result is invalid, and this sample need to be tested again. |
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Feline Serum Amyloid A protein (fSAA) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG013 | EnoGene | 10 Tests/Kit | EUR 56 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Parvo Virus Antigen (CPV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG024 | EnoGene | 10 Tests/Kit | EUR 28 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Feline Plague Virus Antigen (FPV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG021 | EnoGene | 10 Tests/Kit | EUR 38.5 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Feline Herpes Virus Antigen (FHV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG022 | EnoGene | 10 Tests/Kit | EUR 59.5 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Feline Corona Virus Antigen (FCoV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG023 | EnoGene | 10 Tests/Kit | EUR 70 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Corona Virus Antigen (CCV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG026 | EnoGene | 10 Tests/Kit | EUR 30.1 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Thyroid Hormone (T4) Test Kit (Fluorescence Lateral Flow Immunoassay) , For Canine/Feline |
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| HG019 | EnoGene | 10 Tests/Kit | EUR 70 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Distemper Virus Antigen (CDV Ag) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG025 | EnoGene | 10 Tests/Kit | EUR 31.5 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Canine Thyroid-stimulating Hormone (TSH) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG018 | EnoGene | 10 Tests/Kit | EUR 70 |
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Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Feline N-terminal pro-brain nitric peptide (fNT-ProBNP) Test Kit (Fluorescence Lateral Flow Immunoassay) |
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| HG015 | EnoGene | 10 Tests/Kit | EUR 45.5 |
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Description: Contents: (1) 10 Tests/Kit (cass ette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
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Human Proinsulin Immunoassay Kit |
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| MBS398104-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rat Prl(Prolactin) ELISA Kit |
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| ER0076 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.469 ng/ml |
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Human PM20D1 Immunoassay Kit |
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| MBS398105-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse PM20D1 Immunoassay Kit |
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| MBS398109-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Goat PRL(Prolactin) ELISA Kit |
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| EG0008 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Goat;Sensitivity: 18.75IU/ml |
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Ovine insulin Immunoassay Kit |
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| MBS398116-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Human PRL(Prolactin) ELISA Kit |
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| EH0259 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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Mouse PRL(Prolactin) ELISA Kit |
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| EM0158 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
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Sheep PRL(prolactin) ELISA Kit |
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| ESH0063 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 0.188 ng/ml |
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Canine insulin Immunoassay Kit |
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| MBS398114-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Equine insulin Immunoassay Kit |
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| MBS398115-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Bovine PRL(Prolactin) ELISA Kit |
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| EB0013 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 0.469 ng/ml |
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Rabbit PRL(Prolactin) ELISA Kit |
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| ERB0102 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.188 ng/ml |
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Chicken PRL(Prolactin) ELISA Kit |
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| ECH6395 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 0.469 ng/ml |
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Porcine PRL(Prolactin) ELISA Kit |
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| EP0139 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.094 ng/ml |
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Human Autotaxin Immunoassay Kit |
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| MBS398106-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse Autotaxin Immunoassay Kit |
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| MBS398111-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Porcine insulin Immunoassay Kit |
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| MBS398113-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse IL-33 Immunoassay Kit |
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| MBS398110-INQUIRE | MyBiosource | INQUIRE | Ask for price |
(1-Kit) 11,12-DHET Immunoassay Kit |
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| DH4 | Detroit R&D | 1 Kit | EUR 414 |
Human TIMP-1 Immunoassay Kit |
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| MBS398101-96Tests | MyBiosource | 96Tests | EUR 550 |
Human GDF-15 Immunoassay Kit |
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| MBS398108-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse GDF-15 Immunoassay Kit |
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| MBS398112-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse Macrophage Inflammatory Protein 1 BetaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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| IMSMIP1BKTC | Innovative research | each | EUR 545 |
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Description: Mouse Macrophage Inflammatory Protein 1 BetaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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Mouse Prl8a8( Prolactin-8A8) ELISA Kit |
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| EM0798 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml |
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Human Haptoglobin Immunoassay Kit |
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| MBS398103-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Immunoassay Stabilizer |
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| 85R-103 | Fitzgerald | 1 liter | EUR 417.9 |
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Description: Stabilizing buffer for use in immunoassays |
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Immunoassay Stabilizer |
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| MBS539622-1L | MyBiosource | 1L | EUR 680 |
Immunoassay Stabilizer |
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| MBS539622-5x1L | MyBiosource | 5x1L | EUR 2950 |
Human C-peptide Immunoassay Kit |
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| MBS398107-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Mouse Monokine induced by interferon-gammaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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| IMSCXCL9KTC | Innovative research | each | EUR 545 |
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Description: Mouse Monokine induced by interferon-gammaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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Total T4 Immunoassay |
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| MBS480881-1Kit | MyBiosource | 1Kit | EUR 485 |
Mouse Prl3b1(Prolactin 3B1) ELISA Kit |
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| EM1597 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
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Mouse Prl3d1(Prolactin 3D1) ELISA Kit |
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| EM1598 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
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(1-Kit )11,12-EET/DHET Immunoassay Kit |
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| DH5 | Detroit R&D | 1 Kit | EUR 414 |
Control FD Immunoassay |
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| K063M-6 | Audit MicroControls | 6 x 3 mL | EUR 175 |
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Description: Tri-Level |
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Human PRLR(Prolactin receptor) ELISA Kit |
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| EH15147 | FN Test | 96T | EUR 628.92 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human Mothers Against Decapentaplegic Homolog 4 Chemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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| IHUSMAD4KTC | Innovative research | each | EUR 545 |
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Description: Human Mothers Against Decapentaplegic Homolog 4 Chemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit |
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CaspSELECT Caspase 3 Immunoassay Kit |
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| 55R-1338 | Fitzgerald | 100 assays | EUR 860 |
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Description: Assay Kit for detection of Capase 3 activity in the research laboratory |
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Recombinant RAT Prolactin |
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| P1986 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for RAT Prolactin |
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Results of elevated CO 2 remedy of Populus davidiana × P. bolleana on progress and detoxifying enzymes in gypsy moth, Lymantria dispar
To this point, elevated CO2 concentrations within the setting triggered by numerous human actions affect various areas of life, together with the interactions between bugs and crops. The Lymantria dispar is without doubt one of the most severely damaging pests, which additional might inflict ecological and economical injury. On this experiment, one-year-old Populus davidiana × P. bolleana crops had been grown in CO2-enhanced environments for one month at three completely different CO2 concentrations: 397 ppm (atmospheric CO2 focus), 550 ppm and 750 ppm (two predicted elevated CO2 concentrations).
The third instar L. dispar larvae then ate up the handled poplar seedlings coated in a nylon bag. The L. dispar larvae ate up poplar seedling handled for 96 h confirmed the very best progress charge in any respect CO2 concentrations. Enzymatic exercise of handled larvae confirmed the very best GST and P450 exercise at 750 ppm CO2. The relative expressions of seven CYP and ten GST genes in L. dispar larvae had been analyzed quantitatively utilizing real-time RT-PCR, which the outcomes had been expressed variably. In comparison with 397 ppm CO2, the expression of CYP4L23 was down-regulated, whereas the expressions of different CYP genes had been up-regulated.
In the meantime, solely GSTo1 gene confirmed down-regulated at 48 h and 96 h in 750 ppm CO2 remedy, whereas GST expression stage for the opposite 9 GST genes confirmed up-regulated at 48 h and 72 h. These outcomes provide the perception into plant-insect interactions underneath world local weather change and moreover will present important info for strategic pest management based mostly on biochemical and molecular ranges modifications in gypsy moths.
Inhibition of Angiotensin-I Changing Enzyme by Ginsenosides: Construction-Exercise Relationships and Inhibitory Mechanism
Ginseng (Panax ginseng C. A. Meyer) extract has been reported to inhibit the angiotensin changing enzyme (ACE); nevertheless, the doable inhibitory motion of most of its constituents (ginsenosides) in opposition to ACE stays unknown. Thus, on this research, we investigated ginsenoside derivatives’ inhibitory impact on ACE. We assessed the actions of 22 ginsenosides, most of which inhibited ACE considerably. Notably, protopanaxatriol, protopanaxadiol, and ginsenoside Rh2 exhibited probably the most potent ACE inhibitory potential, with IC50 values of 1.57, 2.22, and 5.60 μM, respectively. Additional, a kinetic research revealed completely different modes of inhibition in opposition to ACE.
Molecular docking research have confirmed that ginsenosides inhibit ACE through many hydrogen bonds and hydrophobic interactions with catalytic residues and zinc ion of C- and N-domain ACE that block the catalytic exercise of ACE. As well as, we discovered that the lively ginsenosides stimulated glucose uptake in insulin-resistant C2C12 skeletal muscle cells in a dose-dependent method. Furthermore, probably the most lively ginsenosides’ reactive oxygen species (ROS) and peroxynitrite (ONOO–) scavenging properties had been evaluated, by which IC50 values ranged from 1.44-43.83 to 2.36-39.56 μM in ONOO– and ROS, respectively. The outcomes derived from these computational and in vitro experiments present further scientific help for the anecdotal use of ginseng in conventional medication to deal with cardiovascular ailments equivalent to hypertension.
Selective Motion Mechanism of Fenclorim on Rice and Echinochloa crusgalli Is Related to the Inducibility of Detoxifying Enzyme Actions and Antioxidative Protection
Fenclorim (Fen) is a safener developed for pretilachlor (Pre) that may defend rice from damage attributable to Pre however doesn’t decrease the weed management results of Pre. Sadly, the mechanism of selective motion of Fen between rice and weeds, equivalent to Echinochloa crusgalli (barnyard grass), has not been clarified. On this research, the variations in physiology, biochemistry, and gene transcription between rice and E. crusgalli response to Fen had been in contrast. Evaluating the safety results of Fen on plant progress, it was discovered that Fen considerably protected rice from Pre, however didn’t defend E. crusgalli.
The detection of malondialdehyde (MDA) content material and actions of antioxidant enzymes confirmed that Pre induced vital oxidative injury each in rice and E. crusgalli; nevertheless, Fen lowered oxidative injury in rice however not in E. crusgalli.
Transcriptome evaluation revealed that Fen induced extra genes associated to herbicide metabolism in rice than in E. crusgalli, particularly the glutathione-S-transferase (GST) genes, with six upregulated in rice however no genes upregulated in E. crusgalli. Accordingly, the GST exercise evaluation confirmed that Fen elevated the exercise of rice as an alternative of E. crusgalli. These outcomes point out that the elevation of detoxifying enzyme actions and antioxidative protection will be the mechanism of selective motion of Fen in rice however not in E. crusgalli.
Diagnosing Antibiotic Resistance Utilizing Nucleic Acid Enzymes and Gold Nanoparticles
The speedy and correct detection of antimicrobial resistance is crucial to limiting the unfold of infections and delivering efficient therapies. Right here, we developed a speedy, delicate, and easy colorimetric nanodiagnostic platform to determine disease-causing pathogens and their related antibiotic resistance genes inside 2 h. The platform can detect micro organism from completely different organic samples (i.e., blood, wound swabs) with or with out culturing. We validated the multicomponent nucleic acid enzyme-gold nanoparticle (MNAzyme-GNP) platform by screening sufferers with central line related bloodstream infections and achieved a medical sensitivity and specificity of 86% and 100%, respectively.
We detected antibiotic resistance in methicillin-resistant Staphylococcus aureus (MRSA) in affected person swabs with 90% medical sensitivity and 95% medical specificity. Lastly, we recognized mecA resistance genes in uncultured nasal, groin, axilla, and wound swabs from sufferers with 90% medical sensitivity and 95% medical specificity. The simplicity and flexibility for detecting micro organism and antibiotic resistance markers make our platform enticing for the broad screening of microbial pathogens.
