a488, Multimodal fluorescently labeled polymer-coated GdF 3 nanoparticles inhibit degranulation in mast cells

Multimodal fluorescently labeled polymer-coated GdF 3 nanoparticles inhibit degranulation in mast cells

Multimodal gadolinium fluoride nanoparticles belong to potential distinction brokers helpful for bimodal optical fluorescence and magnetic resonance imaging. Nevertheless, the metallic nature of the nanoparticles, equally to some paramagnetic iron oxides, would possibly induce allergic and anaphylactic reactions in sufferers after administration. A discount of those opposed uncomfortable side effects is a precedence for the secure utility of the nanoparticles.
Herein, we ready paramagnetic poly(4-styrenesulfonic acid-co-maleic acid) (PSSMA)-stabilized GdF3 nanoparticles with floor modified by Atto 488-labeled poly(styrene-grad-2-dimethylaminoethyl acrylate)-block-poly(2-dimethylaminoethyl acrylate) (PSDA-A488) with reactive amino teams for introduction of a further imaging (luminescence) modality and doable focusing on of anticancer medicine.
The saturation magnetization of GdF3@PSSMA particles in response to SQUID magnetometry reached 157 Am2 kg-1 at 2 Okay and magnetic subject of seven T. GdF3@PSSMA-PSDA-A488 nanoparticles have been nicely tolerated by human cervical adenocarcinoma (HeLa), mouse bone marrow-derived mast cells (BMMC), and rat basophilic mast cells (RBL-2H3); the particles additionally affected cell morphology and protein tyrosine phosphorylation in mast cells.
Furthermore, the nanoparticles interfered with the activation of mast cells by multivalent antigens and inhibited calcium mobilization and cell degranulation. These findings present that the brand new multimodal GdF3-based nanoparticles possess properties helpful for numerous imaging strategies and would possibly reduce mast cell degranulation incurred after future nanoparticle diagnostic administration.

Structural Stability and Conformational Dynamics of Cytochrome c in Hydrated Deep Eutectic Solvents

Many deep eutectic solvents (DESs) are at the moment being explored as environment-friendly media for biorelated purposes. As an understanding of the impact of those solvents on the construction of biomolecules is essential for these purposes, we examine how two DESs comprising trimethylglycine (TMG) and ethylene glycol (EG) or glycerol (GL) affect the structural stability and conformational dynamics of cytochrome c (Cytc) utilizing single-molecule-based fluorescence correlation spectroscopy (FCS) approach and a number of other different ensemble-based biophysical strategies.
The FCS research on A488-labeled Cytc allow an estimation of the scale (20.5 ± 1.5 Å) of the protein and seize its conformational dynamics (54 ± 2 μs) in aqueous buffered answer. It’s noticed that each dimension and conformational dynamics of the protein are influenced within the presence of the DESs, however this impact is extra pronounced within the case of TMG-EG.
The ensemble measurements on each labeled and wild-type Cytc reveal that the protein construction is unfolded fully by TMG-EG, whereas the construction is barely altered by TMG-GL. The outcomes counsel that the conduct of Cytc in hydrated DESs is set by the energy of interactions between the DES constituents in addition to that between the constituents and the water molecules current within the system.

Subdural haematomas drain into the extracranial lymphatic system via the meningeal lymphatic vessels.

Subdural haematomas (SDHs) are characterised by quickly or steadily collected haematomas between the arachnoid and dura mater. The mechanism of haematoma clearance has not been clearly elucidated till now. The meningeal lymphatic vessel (mLV) drainage pathway is a novel system that takes half within the clearance of waste merchandise within the central nervous system (CNS).
This examine aimed to discover the roles of the mLV drainage pathway in SDH clearance and its impacting elements. We injected FITC-500D, A488-fibrinogen and autologous blood into the subdural house of mice/rats and located that these substances drained into deep cervical lymph nodes (dCLNs). FITC-500D was additionally noticed within the lymphatic vessels (LYVE+) of the meninges and the dCLNs in mice.
The SDH clearance fee in SDH rats that obtained deep cervical lymph vessel (dCLV) ligation surgical procedure was considerably decrease than that within the management group, as evaluated by haemoglobin quantification and MRI scanning.
The drainage fee of mLVs was considerably slower after the SDH mannequin was established, and the expression of lymphangiogenesis-related proteins, together with LYVE1, FOXC2 and VEGF-C, in meninges was downregulated. In abstract, our findings proved that SDH was absorbed via the mLV drainage pathway and that haematomas might inhibit the operate of mLVs.

Reversible energy-transfer switching on a DNA scaffold.

We present that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently hooked up to a DNA scaffold could be reversibly managed by photochromic switching of a spiropyran by-product. With the spiropyran within the closed spiro isomeric type, FRET happens freely between PB and A488.
UV-induced isomerization to the open merocyanine type shuts down the FRET course of by environment friendly quenching of the PB excited state. The method is reversed by publicity to seen gentle, triggering the isomerization to the spiro isomer.
a488, Multimodal fluorescently labeled polymer-coated GdF 3 nanoparticles inhibit degranulation in mast cells

Rule-based classification fashions of molecular autofluorescence.

Fluorescence-based detection has been generally utilized in high-throughput screening (HTS) assays. Autofluorescent compounds, which may emit gentle within the absence of synthetic fluorescent markers, typically intervene with the detection of fluorophores and end in false optimistic indicators in these assays. This interference presents a serious situation in fluorescence-based screening strategies.
In an effort to cut back the time and price that will likely be spent on prescreening of autofluorescent compounds, in silico autofluorescence prediction fashions have been developed for chosen fluorescence-based assays on this examine. 5 prediction fashions have been developed primarily based on the respective fluorophores utilized in these HTS assays, which take in and emit gentle at particular wavelengths (excitation/emission): Alexa Fluor 350 (A350) (340 nm/450 nm), 7-amino-4-trifluoromethyl-coumarin (AFC) (405 nm/520 nm), Alexa Fluor 488 (A488) (480 nm/540 nm), Rhodamine (547 nm/598 nm), and Texas Pink (547 nm/618 nm).
The C5.zero rule-based classification algorithm and PubChem 2D chemical construction fingerprints have been used to develop prediction fashions. To optimize the accuracies of those prediction fashions regardless of the extremely imbalanced ratio of fluorescent versus nonfluorescent compounds offered within the collected knowledge units, oversampling and undersampling methods have been utilized.

Monoclonal antibody for HSP70/HSC70

SMC-106B-A488 0.2mg
EUR 453
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
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Description: A monoclonal antibody from clone BB70 against Human | Mouse | Rat | Beluga (Delphinapterus leucas) | Bovine | Dog | Fish | Carp (Cypriniformes) | Guinea Pig (Cavia porcellus) | Hamster | Monkey | Pig | Sheep | Coral (Anthozoa) | Plant | Tomato | Tobacco | Shark | Spiny Dogfish Shark (Squalus acanthias) | Atlantic Hagfish (Myxine glutinosa) HSP70/HSC70. The host species for the production of this antibody is Mouse. The antigen used for immunization is Chicken Chicken HSP70/HSP90 complex. The antibody is tested and validated for WB, ICC/IF, IHC, IP assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This MAb for HSP70/HSC70 is conjugated with ATTO 488.

Monoclonal antibody for HSP90

SMC-107B-A488 0.2mg
EUR 453
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). D
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Description: A monoclonal antibody from clone H9010 against Human | Mouse | Rat HSP90. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90beta. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, AM assays with the following recommended dilutions: WB (1:2500), IHC (1:100). This MAb for HSP90 is conjugated with ATTO 488.

Monoclonal antibody for HSP90 alpha

SMC-108D-A488 0.1mg
EUR 412
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). D
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Description: A monoclonal antibody from clone Hyb-K41009 against Human | Mouse | Rat | Bovine | Dog | Chicken | Rabbit | Hamster HSP90 alpha. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90alpha; Specificity mapped to amino acids 604-731. The antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:5000). This MAb for HSP90 alpha is conjugated with ATTO 488.

Monoclonal antibody for HSP90 complex

SMC-109B-A488 0.2mg
EUR 540
  • HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral componen
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Description: A monoclonal antibody from clone 8D3 against Human | Mouse | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) | Dog HSP90 complex. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Ah receptor (Aryl hydrocarbon receptor). The antibody is tested and validated for IP, ICC/IF assays with the following recommended dilutions: ICC/IF (1:100), IP (1:1000). This MAb for HSP90 complex is conjugated with ATTO 488.

Monoclonal antibody for HSP60

SMC-110B-A488 0.2mg
EUR 377
  • In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the b
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Description: A monoclonal antibody from clone LK1 against Human | Mouse | Rat | Bovine | Fungi | Chestnut Blight (Cryphonectria parasitica) | Hamster | Monkey | Rabbit | African clawed frog (Xenopus laevis) | Dog HSP60. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP60. The antibody is tested and validated for WB, IHC, IP, ELISA, FCM assays with the following recommended dilutions: WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200). This MAb for HSP60 is conjugated with ATTO 488.

Monoclonal antibody for HSP60

SMC-111B-A488 0.2mg
EUR 416
  • In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the b
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Description: A monoclonal antibody from clone LK2 against Human | Mouse | Rat | Bovine | Monkey | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Pig | Avian | Quail | Rabbit | Sheep | Fruit Fly (Drosophila melanogaster) | African clawed frog (Xenopus laevis) HSP60. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP60. The antibody is tested and validated for WB, IHC, FCM assays with the following recommended dilutions: WB (1:4000), IHC (1:100). This MAb for HSP60 is conjugated with ATTO 488.

Monoclonal antibody for HSP90

SMC-112B-A488 0.2mg
EUR 453
  • HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral componen
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Description: A monoclonal antibody from clone AC-16 against Human | Mouse | Rat HSP90. The host species for the production of this antibody is Mouse. The antigen used for immunization is Water Mold Heat shock protein 90 from the water mold Achyla ambisexualis. The antibody is tested and validated for WB, IHC, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:2000). This MAb for HSP90 is conjugated with ATTO 488.

Monoclonal antibody for HSP70

SMC-113B-A488 0.2mg
EUR 453
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
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Description: A monoclonal antibody from clone 1.86 against Human | Mouse | Rat | Dog HSP70. The host species for the production of this antibody is Mouse. The antigen used for immunization is Bovine Bovine HSP70. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:100). This MAb for HSP70 is conjugated with ATTO 488.

Monoclonal antibody for HSP25/HSP27

SMC-114D-A488 0.1mg
EUR 355
  • HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid
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Description: A monoclonal antibody from clone 8A7 against Human | Mouse | Rat HSP25/HSP27. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HSP27 peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, FCM, FACS assays with the following recommended dilutions: WB (1:5000), ICC/IF (1:200), IHC (1:100). This MAb for HSP25/HSP27 is conjugated with ATTO 488.

Monoclonal antibody for CD74

SMC-116D-A488 0.1mg
EUR 342
  • CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to fu
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Description: A monoclonal antibody from clone PIN.1 against Human | Mouse | Rat | Bovine | Dog | Hamster | Monkey | Guinea Pig (Cavia porcellus) | Sheep | Pig | African clawed frog (Xenopus laevis) | Mollusk | Mussel (Perna viridis) CD74. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human CD74 invariant chain synthetic peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, FCM, FACS assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:50). This MAb for CD74 is conjugated with ATTO 488.

Monoclonal antibody for HSF1

SMC-118D-A488 0.1mg
EUR 332
  • HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of
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Description: A monoclonal antibody from clone 10H8 against Human | Rat | Mouse | Bovine | Fungi HSF1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, GS assays with the following recommended dilutions: WB (1:1000), IHC (1:1000), ICC/IF (1:200). This MAb for HSF1 is conjugated with ATTO 488.

Monoclonal antibody for HSF2

SMC-119D-A488 0.1mg
EUR 332
  • HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of
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Description: A monoclonal antibody from clone 3E2 against Human | Rat | Mouse | Bovine | Monkey | Invertebrate | Coral (Anthozoa) | Dog | Hamster | Pig | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Mollusk | Mussel (Perna viridis) | Fish (O. melastigma) HSF2. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified recombinant mouse HSF2 protein. The antibody is tested and validated for WB, ICC/IF, AM assays with the following recommended dilutions: WB (1:250), ICC/IF (1:200). This MAb for HSF2 is conjugated with ATTO 488.

Monoclonal antibody for HSP70/HSC70 (Plant)

SMC-120D-A488 0.1mg
EUR 378
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
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Description: A monoclonal antibody from clone 5G1-95 against Human | Rat | Mouse | Bovine | Dog | Chicken | Fruit Fly (Drosophila melanogaster) | Guinea Pig (Cavia porcellus) | Pig | Hamster | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Squirrel | Invertebrate | Sea squirt (Ciona intestinalis) HSP70/HSC70 (Plant). The host species for the production of this antibody is Mouse. The antigen used for immunization is Plant Purified HSP70 from Phaseolus aureus (mung bean). The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000). This MAb for HSP70/HSC70 (Plant) is conjugated with ATTO 488.

Monoclonal antibody for PSD95

SMC-122D-A488 0.1mg
EUR 378
  • Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function o
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Description: A monoclonal antibody from clone 6G6 against Human | Rat | Mouse | Bovine | Dog | Chicken | Gerbil | Guinea Pig (Cavia porcellus) | Pig | Hamster | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Frog | Wood Frog (Rana sylvatica) PSD95. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Recombinant rat PSD-95. The antibody is tested and validated for WB, IHC, ICC/IF, AM assays with the following recommended dilutions: WB (1:250), IHC (1:1000), ICC/IF (1:100). This MAb for PSD95 is conjugated with ATTO 488.

Monoclonal antibody for PSD95

SMC-123D-A488 0.1mg
EUR 378
  • Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function o
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Description: A monoclonal antibody from clone 7E3 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Rabbit | Guinea Pig (Cavia porcellus) | Pig | Dog | Sheep | Chicken | African clawed frog (Xenopus laevis) | Yeast | Fruit Fly (Drosophila melanogaster) | Fish | Rainbow Trout (Oncorhynchus mykiss) PSD95. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Recombinant rat PSD-95. The antibody is tested and validated for WB, IHC, ICC/IF, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:1000), ICC/IF (1:100). This MAb for PSD95 is conjugated with ATTO 488.

Monoclonal antibody for CaMKII (Alpha-Specific)

SMC-124D-A488 0.1mg
EUR 378
  • CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the n
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Description: A monoclonal antibody from clone 6G9 against Human | Mouse | Rat | Bovine | Sheep | Chicken | Fruit Fly (Drosophila melanogaster) | African clawed frog (Xenopus laevis) CaMKII (Alpha-Specific). The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Partially purified rat CaMKII. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, RIA assays with the following recommended dilutions: WB (1:10000), IHC (1:2000), ICC/IF (1:50). This MAb for CaMKII (Alpha-Specific) is conjugated with ATTO 488.

Monoclonal antibody for CaMKII

SMC-125D-A488 0.1mg
EUR 378
  • CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the n
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Description: A monoclonal antibody from clone 22B1 against Human | Mouse | Rat | Bovine | Dog | Chicken | Guinea Pig (Cavia porcellus) | Monkey | Pig | Hamster | Rabbit | Sheep CaMKII. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000). This MAb for CaMKII is conjugated with ATTO 488.

Monoclonal antibody for KDEL Receptor

SMC-129D-A488 0.1mg
EUR 385
  • The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention mot
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Description: A monoclonal antibody from clone KR-10 against Human | Mouse | Rat KDEL Receptor. The host species for the production of this antibody is Mouse. The antigen used for immunization is Bovine A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH. The antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:1000). This MAb for KDEL Receptor is conjugated with ATTO 488.

Monoclonal antibody for HO-1

SMC-131D-A488 0.1mg
EUR 403
  • Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is seq
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Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 488.

Monoclonal antibody for Grp75

SMC-133D-A488 0.1mg
EUR 395
  • Grp75, also known as mortalin, is a member of HSP70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in
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Description: A monoclonal antibody from clone S52A-42 against Human | Mouse | Rat | Bovine | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Pig | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) Grp75. The host species for the production of this antibody is Mouse. The antigen used for immunization is Mouse Fusion protein amino acids 551-766 of mouse SALM2.. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:200). This MAb for Grp75 is conjugated with ATTO 488.

Monoclonal antibody for SAP102

SMC-134D-A488 0.1mg
EUR 339
  • Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that faci
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Description: A monoclonal antibody from clone S19-2 against Human | Mouse | Rat SAP102. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Fusion protein amino acids 1-120 of rat SAP102. The antibody is tested and validated for WB, IHC, IP, ICC/IF, AM assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for SAP102 is conjugated with ATTO 488.

Monoclonal antibody for HSP90 alpha/beta

SMC-135D-A488 0.1mg
EUR 412
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). De
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Description: A monoclonal antibody from clone Hyb-K41220A against Human HSP90 alpha/beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304. The antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HSP90 alpha/beta is conjugated with ATTO 488.

Monoclonal antibody for HSP90 beta

SMC-136D-A488 0.1mg
EUR 412
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). De
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Description: A monoclonal antibody from clone Hyb-K3701 against Human | Mouse HSP90 beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90beta; Specificity mapped to amino acids 185-335. The antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:3000). This MAb for HSP90 beta is conjugated with ATTO 488.

Monoclonal antibody for HSP90

SMC-137D-A488 0.1mg
EUR 412
  • HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral componen
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Description: A monoclonal antibody from clone D7A against Human | Mouse | Rat | Bovine HSP90. The host species for the production of this antibody is Mouse. The antigen used for immunization is Chicken Full length protein HSP90 purified from chicken brain. The antibody is tested and validated for WB, IHC, IP, ELISA, AM assays with the following recommended dilutions: WB (1:500), IP (5µg) . This MAb for HSP90 is conjugated with ATTO 488.

Monoclonal antibody for FKBP51

SMC-138D-A488 0.1mg
EUR 412
  • HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone
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Description: A monoclonal antibody from clone Hi51B against Human | Mouse | Rat | Bovine FKBP51. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Synthetic peptide corresponding to the residues of human FKBP51. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:1000). This MAb for FKBP51 is conjugated with ATTO 488.

Monoclonal antibody for FKBP52

SMC-139D-A488 0.1mg
EUR 412
  • HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone
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Description: A monoclonal antibody from clone Hi52C against Human | Mouse | Rat | Bovine | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Monkey | Pig | Rabbit | Sheep | African clawed frog (Xenopus laevis) FKBP52. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Synthetic peptide corresponding to the residues of human FKBP52. The antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg). This MAb for FKBP52 is conjugated with ATTO 488.

Monoclonal antibody for LAMP1

SMC-140D-A488 0.1mg
EUR 390
  • Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primaril
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Description: A monoclonal antibody from clone Ly1C6 against Species Independent LAMP1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Rat liver lysosomal membrane preparations. The antibody is tested and validated for WB, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:1000). This MAb for LAMP1 is conjugated with ATTO 488.

Monoclonal antibody for LAMP2

SMC-141D-A488 0.1mg
EUR 379
  • Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primaril
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Description: A monoclonal antibody from clone GL2A7 against Species Independent LAMP2. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified preparation of mouse liver lysosomal membranes. The antibody is tested and validated for WB, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:500). This MAb for LAMP2 is conjugated with ATTO 488.

Monoclonal antibody for Alpha A Crystallin

SMC-142B-A488 0.2mg
EUR 408
  • The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mamma
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Description: A monoclonal antibody from clone 1H3.B8 against Species Independent Alpha A Crystallin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Native Alpha Crystallin. The antibody is tested and validated for WB, ELISA, ICC/IF assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for Alpha A Crystallin is conjugated with ATTO 488.

Monoclonal antibody for HSP40, Hdj1

SMC-145D-A488 0.1mg
EUR 368
  • Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/ph
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Description: A monoclonal antibody from clone 3B9.E6 against Species Independent HSP40, Hdj1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant Protein HSP40 (Hdj1). The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for HSP40, Hdj1 is conjugated with ATTO 488.

Monoclonal antibody for HSP90 alpha

SMC-147B-A488 0.2mg
EUR 453
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). De
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Description: A monoclonal antibody from clone 2G5.G3 against Species Independent HSP90 alpha. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HSP90alpha. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for HSP90 alpha is conjugated with ATTO 488.

Monoclonal antibody for HSP90 (total)

SMC-149B-A488 0.2mg
EUR 453
  • HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms ? and ?, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). De
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Description: A monoclonal antibody from clone 4F3.E8 against Human | Mouse | Rat HSP90 (total). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant Human HSP90 purified from E.coli. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), IHC (1:100), ICC/IF (1:100). This MAb for HSP90 (total) is conjugated with ATTO 488.

Monoclonal antibody for HSP40, YDJ1

SMC-150D-A488 0.1mg
EUR 393
  • Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/ph
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Description: A monoclonal antibody from clone 1G10.H8 against Human | Rat | Rainbow Trout (Oncorhynchus mykiss) HSP40, YDJ1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Yeast Full length protein yeast HSP40 (YDJ1). The antibody is tested and validated for WB, IP, ELISA assays with the following recommended dilutions: WB (1:2000). This MAb for HSP40, YDJ1 is conjugated with ATTO 488.

Monoclonal antibody for HSC70 (HSP73)

SMC-151B-A488 0.2mg
EUR 468
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
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Description: A monoclonal antibody from clone 1F2-H5 against Human | Mouse | Chicken HSC70 (HSP73). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length human HSC70. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, PLA, PBA, AM assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for HSC70 (HSP73) is conjugated with ATTO 488.

Monoclonal antibody for p38 alpha (MAP Kinase)

SMC-152D-A488 0.1mg
EUR 343
  • The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major devel
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Description: A monoclonal antibody from clone 9F12 against Human | Mouse | Rat p38 alpha (MAP Kinase). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length recombinant protein expressed in E.coli cells. The antibody is tested and validated for WB, IHC, IP, ELISA, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:1000). This MAb for p38 alpha (MAP Kinase) is conjugated with ATTO 488.
The common last accuracy achieved for the coaching set was 97%, and that for the testing set was 92%. As well as, 5 exterior knowledge units have been used to additional validate the fashions. In the end, 14 consultant structural options (or guidelines) have been decided to effectively predict autofluorescence in knowledge units containing each fluorescent and nonfluorescent compounds. A number of circumstances have been illustrated on this examine to show the applicability of those guidelines.

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