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Multimodal fluorescently labeled polymer-coated GdF 3 nanoparticles inhibit degranulation in mast cells
Multimodal gadolinium fluoride nanoparticles belong to potential distinction brokers helpful for bimodal optical fluorescence and magnetic resonance imaging. Nevertheless, the metallic nature of the nanoparticles, equally to some paramagnetic iron oxides, would possibly induce allergic and anaphylactic reactions in sufferers after administration. A discount of those opposed uncomfortable side effects is a precedence for the secure utility of the nanoparticles.
Herein, we ready paramagnetic poly(4-styrenesulfonic acid-co-maleic acid) (PSSMA)-stabilized GdF3 nanoparticles with floor modified by Atto 488-labeled poly(styrene-grad-2-dimethylaminoethyl acrylate)-block-poly(2-dimethylaminoethyl acrylate) (PSDA-A488) with reactive amino teams for introduction of a further imaging (luminescence) modality and doable focusing on of anticancer medicine.
The saturation magnetization of GdF3@PSSMA particles in response to SQUID magnetometry reached 157 Am2 kg-1 at 2 Okay and magnetic subject of seven T. GdF3@PSSMA-PSDA-A488 nanoparticles have been nicely tolerated by human cervical adenocarcinoma (HeLa), mouse bone marrow-derived mast cells (BMMC), and rat basophilic mast cells (RBL-2H3); the particles additionally affected cell morphology and protein tyrosine phosphorylation in mast cells.
Furthermore, the nanoparticles interfered with the activation of mast cells by multivalent antigens and inhibited calcium mobilization and cell degranulation. These findings present that the brand new multimodal GdF3-based nanoparticles possess properties helpful for numerous imaging strategies and would possibly reduce mast cell degranulation incurred after future nanoparticle diagnostic administration.
Structural Stability and Conformational Dynamics of Cytochrome c in Hydrated Deep Eutectic Solvents
Many deep eutectic solvents (DESs) are at the moment being explored as environment-friendly media for biorelated purposes. As an understanding of the impact of those solvents on the construction of biomolecules is essential for these purposes, we examine how two DESs comprising trimethylglycine (TMG) and ethylene glycol (EG) or glycerol (GL) affect the structural stability and conformational dynamics of cytochrome c (Cytc) utilizing single-molecule-based fluorescence correlation spectroscopy (FCS) approach and a number of other different ensemble-based biophysical strategies.
The FCS research on A488-labeled Cytc allow an estimation of the scale (20.5 ± 1.5 Å) of the protein and seize its conformational dynamics (54 ± 2 μs) in aqueous buffered answer. It’s noticed that each dimension and conformational dynamics of the protein are influenced within the presence of the DESs, however this impact is extra pronounced within the case of TMG-EG.
The ensemble measurements on each labeled and wild-type Cytc reveal that the protein construction is unfolded fully by TMG-EG, whereas the construction is barely altered by TMG-GL. The outcomes counsel that the conduct of Cytc in hydrated DESs is set by the energy of interactions between the DES constituents in addition to that between the constituents and the water molecules current within the system.
Subdural haematomas drain into the extracranial lymphatic system via the meningeal lymphatic vessels.
Subdural haematomas (SDHs) are characterised by quickly or steadily collected haematomas between the arachnoid and dura mater. The mechanism of haematoma clearance has not been clearly elucidated till now. The meningeal lymphatic vessel (mLV) drainage pathway is a novel system that takes half within the clearance of waste merchandise within the central nervous system (CNS).
This examine aimed to discover the roles of the mLV drainage pathway in SDH clearance and its impacting elements. We injected FITC-500D, A488-fibrinogen and autologous blood into the subdural house of mice/rats and located that these substances drained into deep cervical lymph nodes (dCLNs). FITC-500D was additionally noticed within the lymphatic vessels (LYVE+) of the meninges and the dCLNs in mice.
The SDH clearance fee in SDH rats that obtained deep cervical lymph vessel (dCLV) ligation surgical procedure was considerably decrease than that within the management group, as evaluated by haemoglobin quantification and MRI scanning.
The drainage fee of mLVs was considerably slower after the SDH mannequin was established, and the expression of lymphangiogenesis-related proteins, together with LYVE1, FOXC2 and VEGF-C, in meninges was downregulated. In abstract, our findings proved that SDH was absorbed via the mLV drainage pathway and that haematomas might inhibit the operate of mLVs.
Reversible energy-transfer switching on a DNA scaffold.
We present that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently hooked up to a DNA scaffold could be reversibly managed by photochromic switching of a spiropyran by-product. With the spiropyran within the closed spiro isomeric type, FRET happens freely between PB and A488.
UV-induced isomerization to the open merocyanine type shuts down the FRET course of by environment friendly quenching of the PB excited state. The method is reversed by publicity to seen gentle, triggering the isomerization to the spiro isomer.
Rule-based classification fashions of molecular autofluorescence.
Fluorescence-based detection has been generally utilized in high-throughput screening (HTS) assays. Autofluorescent compounds, which may emit gentle within the absence of synthetic fluorescent markers, typically intervene with the detection of fluorophores and end in false optimistic indicators in these assays. This interference presents a serious situation in fluorescence-based screening strategies.
In an effort to cut back the time and price that will likely be spent on prescreening of autofluorescent compounds, in silico autofluorescence prediction fashions have been developed for chosen fluorescence-based assays on this examine. 5 prediction fashions have been developed primarily based on the respective fluorophores utilized in these HTS assays, which take in and emit gentle at particular wavelengths (excitation/emission): Alexa Fluor 350 (A350) (340 nm/450 nm), 7-amino-4-trifluoromethyl-coumarin (AFC) (405 nm/520 nm), Alexa Fluor 488 (A488) (480 nm/540 nm), Rhodamine (547 nm/598 nm), and Texas Pink (547 nm/618 nm).
The C5.zero rule-based classification algorithm and PubChem 2D chemical construction fingerprints have been used to develop prediction fashions. To optimize the accuracies of those prediction fashions regardless of the extremely imbalanced ratio of fluorescent versus nonfluorescent compounds offered within the collected knowledge units, oversampling and undersampling methods have been utilized.
Monoclonal antibody for HSP47 |
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| SMC-203B-A488 | Stressmarq | 0.2mg | EUR 468 |
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Description: A monoclonal antibody from clone 1C4-1A6 against Human | Mouse | Rat | African clawed frog (Xenopus laevis) | Hamster HSP47. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HSP47, full length. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for HSP47 is conjugated with ATTO 488. |
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Monoclonal antibody for EndoPDI |
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| SMC-204D-A488 | Stressmarq | 0.1mg | EUR 399 |
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Description: A monoclonal antibody from clone 2E7/7 against Rat | Mouse | African clawed frog (Xenopus laevis) | Hamster | Human EndoPDI. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:200). This MAb for EndoPDI is conjugated with ATTO 488. |
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Monoclonal antibody for Trap1 |
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| SMC-207D-A488 | Stressmarq | 0.1mg | EUR 390 |
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Description: A monoclonal antibody from clone 3H4-2H6 against Rat | Mouse Trap1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Purified recombinant TRAP1. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for Trap1 is conjugated with ATTO 488. |
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Monoclonal antibody for Alginate |
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| SMC-208D-A488 | Stressmarq | 0.1mg | EUR 419 |
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Description: A monoclonal antibody from clone 4B10-1C5 against Human | Mouse | Rat Alginate. The host species for the production of this antibody is Mouse. The antigen used for immunization is Sodium Alginate conjugated to KLH. The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:500), IHC (1:200). This MAb for Alginate is conjugated with ATTO 488. |
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Monoclonal antibody for Alginate |
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| SMC-209D-A488 | Stressmarq | 0.1mg | EUR 419 |
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Description: A monoclonal antibody from clone 3G4-1F5 against Human | Mouse | Rat Alginate. The host species for the production of this antibody is Mouse. The antigen used for immunization is Sodium Alginate conjugated to KLH. The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:500). This MAb for Alginate is conjugated with ATTO 488. |
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Monoclonal antibody for GRP78 |
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| SMC-210D-A488 | Stressmarq | 0.1mg | EUR 390 |
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Description: A monoclonal antibody from clone 3C5-1A4 against Human | Mouse | Rat GRP78. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Full-length recombinant rat GRP78. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for GRP78 is conjugated with ATTO 488. |
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Monoclonal antibody for GRP78 |
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| SMC-211D-A488 | Stressmarq | 0.1mg | EUR 390 |
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Description: A monoclonal antibody from clone 3G12-1G11 against Human | Mouse | Rat GRP78. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Full-length recombinant rat GRP78. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for GRP78 is conjugated with ATTO 488. |
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Monoclonal antibody for LC3C |
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| SMC-212A-A488 | Stressmarq | 0.05mg | EUR 390 |
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Description: A monoclonal antibody from clone VB2 against Human | Mouse | Rat | Eukaryote LC3C. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Peptide derived from C-terminal region of human LC3C protein. The antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:10000). This MAb for LC3C is conjugated with ATTO 488. |
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Monoclonal antibody for Ubiquitin |
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| SMC-213D-A488 | Stressmarq | 0.1mg | EUR 262 |
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Description: A monoclonal antibody from clone FK1 against Human Ubiquitin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Ubiquitin conjugated lysozyme. The antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:10000), ICC/IF (1:100). This MAb for Ubiquitin is conjugated with ATTO 488. |
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Monoclonal antibody for Ubiquitin |
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| SMC-214D-A488 | Stressmarq | 0.1mg | EUR 262 |
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Description: A monoclonal antibody from clone FK2 against Human | Rat Ubiquitin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Ubiquitin conjugated lysozyme. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:10000). This MAb for Ubiquitin is conjugated with ATTO 488. |
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Monoclonal antibody for Ubiquitin |
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| SMC-215D-A488 | Stressmarq | 0.1ml | EUR 429 |
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Description: A monoclonal antibody from clone Ubi-1 against Human | Rat Ubiquitin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Bovine Bovine ubiquitin, KLH-conjugate. The antibody is tested and validated for WB, IHC, ICC/IF, ELISA
assays with the following recommended dilutions: WB (1:1000), IHC (1:1000), ICC/IF (1:500). This MAb for Ubiquitin is conjugated with ATTO 488. |
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Monoclonal antibody for HSP70 |
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| SMC-230D-A488 | Stressmarq | 0.1mg | EUR 385 |
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Description: A monoclonal antibody from clone 7FB against Human | Rat HSP70. The host species for the production of this antibody is Rat. The antigen used for immunization is Drosophila Prepared from Drosophila tissue culture cells heat shocked at 36.5?C for 3 hours, and isolated using SDS PAGE.. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000). This MAb for HSP70 is conjugated with ATTO 488. |
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Monoclonal antibody for DNMT1 |
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| SMC-231D-A488 | Stressmarq | 0.1mg | EUR 390 |
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Description: A monoclonal antibody from clone 11H8 against Human | Rat DNMT1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for DNMT1 is conjugated with ATTO 488. |
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Monoclonal antibody for GRP170 |
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| SMC-232D-A488 | Stressmarq | 0.1mg | EUR 399 |
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Description: A monoclonal antibody from clone 6E3-2C3 against Human | Mouse GRP170. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length GRP170 protein. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for GRP170 is conjugated with ATTO 488. |
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Monoclonal antibody for GRP170 |
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| SMC-233D-A488 | Stressmarq | 0.1mg | EUR 399 |
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Description: A monoclonal antibody from clone 6G7-2H5 against Human | Mouse | Rat GRP170. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Raised against a synthetic peptide of human GRP170. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for GRP170 is conjugated with ATTO 488. |
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Monoclonal antibody for HO-1 (Rat) |
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| SMC-234D-A488 | Stressmarq | 0.1mg | EUR 399 |
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Description: A monoclonal antibody from clone 6B8-2F2 against Human | Mouse | Rat HO-1 (Rat). The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat His-tagged Rat HO-1. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for HO-1 (Rat) is conjugated with ATTO 488. |
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Monoclonal antibody for ENaC alpha |
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| SMC-239D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 2G4 against Human ENaC alpha. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68). The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:1000); IHC (1:150). This MAb for ENaC alpha is conjugated with ATTO 488. |
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Monoclonal antibody for ENaC beta |
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| SMC-240D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 7B8 against Human ENaC beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638). The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:1000); IHC (1:150). This MAb for ENaC beta is conjugated with ATTO 488. |
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Monoclonal antibody for ENaC beta |
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| SMC-241D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 16E4 against Human | Mouse | Rat ENaC beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638). The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This MAb for ENaC beta is conjugated with ATTO 488. |
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Monoclonal antibody for ENaC alpha |
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| SMC-242D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 14E10 against Human | Mouse | Rat ENaC alpha. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68). The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:1000); IHC (1:150). This MAb for ENaC alpha is conjugated with ATTO 488. |
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Monoclonal antibody for PP5 |
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| SMC-243D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 2E11 against Human PP5. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length human PP5 protein. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for PP5 is conjugated with ATTO 488. |
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Monoclonal antibody for PP5 |
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| SMC-244D-A488 | Stressmarq | 0.1mg | EUR 400 |
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Description: A monoclonal antibody from clone 12F7 against Human PP5. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length human PP5 protein. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for PP5 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP70 |
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| SMC-249D-A488 | Stressmarq | 0.1mg | EUR 454 |
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Description: A monoclonal antibody from clone 1H11 against Human | Mouse | Rat HSP70. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human native HSP70 protein. The antibody is tested and validated for WB, ICC/IF, FCM, FACS assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100), FACS (1:250). This MAb for HSP70 is conjugated with ATTO 488. |
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Monoclonal antibody for Histone H3 (di Methyl Lys79) |
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| SMC-250D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone Q7 against Human | Mouse Histone H3 (di Methyl Lys79). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Prepared by immunizing synthetic peptide coupled to KLH.. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:2000). This MAb for Histone H3 (di Methyl Lys79) is conjugated with ATTO 488. |
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Monoclonal antibody for Histone H3 (tri Methyl Lys36) |
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| SMC-251D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone Q12 against Human | Mouse Histone H3 (tri Methyl Lys36). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Prepared by immunizing synthetic peptide coupled to KLH.. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:5000). This MAb for Histone H3 (tri Methyl Lys36) is conjugated with ATTO 488. |
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Monoclonal antibody for Histone H3 (tri Methyl Lys4) |
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| SMC-252D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 2E11 against Human | Mouse Histone H3 (tri Methyl Lys4). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Prepared by immunizing synthetic peptide coupled to KLH.. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This MAb for Histone H3 (tri Methyl Lys4) is conjugated with ATTO 488. |
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Monoclonal antibody for Histone H3 (tri Methyl Lys79) |
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| SMC-253D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 5D8 against Human | Rat Histone H3 (tri Methyl Lys79). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Prepared by immunizing synthetic peptide coupled to KLH.. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This MAb for Histone H3 (tri Methyl Lys79) is conjugated with ATTO 488. |
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Monoclonal antibody for Histone H3 (tri Methyl Lys9) |
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| SMC-254D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 2C3 against Human | Rat Histone H3 (tri Methyl Lys9). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Prepared by immunizing synthetic peptide coupled to KLH.. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This MAb for Histone H3 (tri Methyl Lys9) is conjugated with ATTO 488. |
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Monoclonal antibody for Myc Tag |
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| SMC-255D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 5D6 against Human | Rat Myc Tag. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human A synthetic peptide EQKLISEEDL coupled to KLH.. The antibody is tested and validated for WB, IP, ICC/IF assays with the following recommended dilutions: WB (1:10000), IP (1:200), ICC/IF (1:1000). This MAb for Myc Tag is conjugated with ATTO 488. |
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Monoclonal antibody for CD45 |
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| SMC-256D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 12A9 against Human | Mouse | Rat CD45. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Purified recombinant fragment of human CD45 expressed in E. Coli. The antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:1000). This MAb for CD45 is conjugated with ATTO 488. |
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Monoclonal antibody for CD68 |
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| SMC-257D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 6F3 against Human CD68. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human A synthesized peptide derived from human CD68. The antibody is tested and validated for IHC assays with the following recommended dilutions: IHC (1:100). This MAb for CD68 is conjugated with ATTO 488. |
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Monoclonal antibody for Myosin heavy chain |
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| SMC-258D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 11C2 against Human | Mouse Myosin heavy chain. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human A synthesized peptide derived from human Myosin Heavy Chain. The antibody is tested and validated for IHC, ICC/IF assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:100). This MAb for Myosin heavy chain is conjugated with ATTO 488. |
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Monoclonal antibody for PARP |
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| SMC-259D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone M3 against Human | Mouse PARP. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human A synthesized peptide derived from human PARP. The antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:2000). This MAb for PARP is conjugated with ATTO 488. |
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Monoclonal antibody for HA tag |
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| SMC-260D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone HA.C5 against Human | Mouse | Rat HA tag. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.. The antibody is tested and validated for WB, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:400), IP (1:400). This MAb for HA tag is conjugated with ATTO 488. |
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Monoclonal antibody for Vimentin |
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| SMC-261D-A488 | Stressmarq | 0.1ml | EUR 360 |
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Description: A monoclonal antibody from clone 1A7 against Human Vimentin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full-length protein.. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:200). This MAb for Vimentin is conjugated with ATTO 488. |
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The common last accuracy achieved for the coaching set was 97%, and that for the testing set was 92%. As well as, 5 exterior knowledge units have been used to additional validate the fashions. In the end, 14 consultant structural options (or guidelines) have been decided to effectively predict autofluorescence in knowledge units containing each fluorescent and nonfluorescent compounds. A number of circumstances have been illustrated on this examine to show the applicability of those guidelines.
