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Multimodal fluorescently labeled polymer-coated GdF 3 nanoparticles inhibit degranulation in mast cells
Multimodal gadolinium fluoride nanoparticles belong to potential distinction brokers helpful for bimodal optical fluorescence and magnetic resonance imaging. Nevertheless, the metallic nature of the nanoparticles, equally to some paramagnetic iron oxides, would possibly induce allergic and anaphylactic reactions in sufferers after administration. A discount of those opposed uncomfortable side effects is a precedence for the secure utility of the nanoparticles.
Herein, we ready paramagnetic poly(4-styrenesulfonic acid-co-maleic acid) (PSSMA)-stabilized GdF3 nanoparticles with floor modified by Atto 488-labeled poly(styrene-grad-2-dimethylaminoethyl acrylate)-block-poly(2-dimethylaminoethyl acrylate) (PSDA-A488) with reactive amino teams for introduction of a further imaging (luminescence) modality and doable focusing on of anticancer medicine.
The saturation magnetization of GdF3@PSSMA particles in response to SQUID magnetometry reached 157 Am2 kg-1 at 2 Okay and magnetic subject of seven T. GdF3@PSSMA-PSDA-A488 nanoparticles have been nicely tolerated by human cervical adenocarcinoma (HeLa), mouse bone marrow-derived mast cells (BMMC), and rat basophilic mast cells (RBL-2H3); the particles additionally affected cell morphology and protein tyrosine phosphorylation in mast cells.
Furthermore, the nanoparticles interfered with the activation of mast cells by multivalent antigens and inhibited calcium mobilization and cell degranulation. These findings present that the brand new multimodal GdF3-based nanoparticles possess properties helpful for numerous imaging strategies and would possibly reduce mast cell degranulation incurred after future nanoparticle diagnostic administration.
Structural Stability and Conformational Dynamics of Cytochrome c in Hydrated Deep Eutectic Solvents
Many deep eutectic solvents (DESs) are at the moment being explored as environment-friendly media for biorelated purposes. As an understanding of the impact of those solvents on the construction of biomolecules is essential for these purposes, we examine how two DESs comprising trimethylglycine (TMG) and ethylene glycol (EG) or glycerol (GL) affect the structural stability and conformational dynamics of cytochrome c (Cytc) utilizing single-molecule-based fluorescence correlation spectroscopy (FCS) approach and a number of other different ensemble-based biophysical strategies.
The FCS research on A488-labeled Cytc allow an estimation of the scale (20.5 ± 1.5 Å) of the protein and seize its conformational dynamics (54 ± 2 μs) in aqueous buffered answer. It’s noticed that each dimension and conformational dynamics of the protein are influenced within the presence of the DESs, however this impact is extra pronounced within the case of TMG-EG.
The ensemble measurements on each labeled and wild-type Cytc reveal that the protein construction is unfolded fully by TMG-EG, whereas the construction is barely altered by TMG-GL. The outcomes counsel that the conduct of Cytc in hydrated DESs is set by the energy of interactions between the DES constituents in addition to that between the constituents and the water molecules current within the system.
Subdural haematomas drain into the extracranial lymphatic system via the meningeal lymphatic vessels.
Subdural haematomas (SDHs) are characterised by quickly or steadily collected haematomas between the arachnoid and dura mater. The mechanism of haematoma clearance has not been clearly elucidated till now. The meningeal lymphatic vessel (mLV) drainage pathway is a novel system that takes half within the clearance of waste merchandise within the central nervous system (CNS).
This examine aimed to discover the roles of the mLV drainage pathway in SDH clearance and its impacting elements. We injected FITC-500D, A488-fibrinogen and autologous blood into the subdural house of mice/rats and located that these substances drained into deep cervical lymph nodes (dCLNs). FITC-500D was additionally noticed within the lymphatic vessels (LYVE+) of the meninges and the dCLNs in mice.
The SDH clearance fee in SDH rats that obtained deep cervical lymph vessel (dCLV) ligation surgical procedure was considerably decrease than that within the management group, as evaluated by haemoglobin quantification and MRI scanning.
The drainage fee of mLVs was considerably slower after the SDH mannequin was established, and the expression of lymphangiogenesis-related proteins, together with LYVE1, FOXC2 and VEGF-C, in meninges was downregulated. In abstract, our findings proved that SDH was absorbed via the mLV drainage pathway and that haematomas might inhibit the operate of mLVs.
Reversible energy-transfer switching on a DNA scaffold.
We present that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently hooked up to a DNA scaffold could be reversibly managed by photochromic switching of a spiropyran by-product. With the spiropyran within the closed spiro isomeric type, FRET happens freely between PB and A488.
UV-induced isomerization to the open merocyanine type shuts down the FRET course of by environment friendly quenching of the PB excited state. The method is reversed by publicity to seen gentle, triggering the isomerization to the spiro isomer.
Rule-based classification fashions of molecular autofluorescence.
Fluorescence-based detection has been generally utilized in high-throughput screening (HTS) assays. Autofluorescent compounds, which may emit gentle within the absence of synthetic fluorescent markers, typically intervene with the detection of fluorophores and end in false optimistic indicators in these assays. This interference presents a serious situation in fluorescence-based screening strategies.
In an effort to cut back the time and price that will likely be spent on prescreening of autofluorescent compounds, in silico autofluorescence prediction fashions have been developed for chosen fluorescence-based assays on this examine. 5 prediction fashions have been developed primarily based on the respective fluorophores utilized in these HTS assays, which take in and emit gentle at particular wavelengths (excitation/emission): Alexa Fluor 350 (A350) (340 nm/450 nm), 7-amino-4-trifluoromethyl-coumarin (AFC) (405 nm/520 nm), Alexa Fluor 488 (A488) (480 nm/540 nm), Rhodamine (547 nm/598 nm), and Texas Pink (547 nm/618 nm).
The C5.zero rule-based classification algorithm and PubChem 2D chemical construction fingerprints have been used to develop prediction fashions. To optimize the accuracies of those prediction fashions regardless of the extremely imbalanced ratio of fluorescent versus nonfluorescent compounds offered within the collected knowledge units, oversampling and undersampling methods have been utilized.
Monoclonal antibody for HSP60 |
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| SMC-110B-A488 | Stressmarq | 0.2mg | EUR 452.4 |
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Description: A monoclonal antibody from clone LK1 against Human | Mouse | Rat | Bovine | Fungi | Chestnut Blight (Cryphonectria parasitica) | Hamster | Monkey | Rabbit | African clawed frog (Xenopus laevis) | Dog HSP60. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP60. The antibody is tested and validated for WB, IHC, IP, ELISA, FCM assays with the following recommended dilutions: WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200). This MAb for HSP60 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP60 |
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| SMC-111B-A488 | Stressmarq | 0.2mg | EUR 499.2 |
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Description: A monoclonal antibody from clone LK2 against Human | Mouse | Rat | Bovine | Monkey | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Pig | Avian | Quail | Rabbit | Sheep | Fruit Fly (Drosophila melanogaster) | African clawed frog (Xenopus laevis) HSP60. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP60. The antibody is tested and validated for WB, IHC, FCM assays with the following recommended dilutions: WB (1:4000), IHC (1:100). This MAb for HSP60 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 |
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| SMC-112B-A488 | Stressmarq | 0.2mg | EUR 543.6 |
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Description: A monoclonal antibody from clone AC-16 against Human | Mouse | Rat HSP90. The host species for the production of this antibody is Mouse. The antigen used for immunization is Water Mold Heat shock protein 90 from the water mold Achyla ambisexualis. The antibody is tested and validated for WB, IHC, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:2000). This MAb for HSP90 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP70 |
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| SMC-113B-A488 | Stressmarq | 0.2mg | EUR 543.6 |
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Description: A monoclonal antibody from clone 1.86 against Human | Mouse | Rat | Dog HSP70. The host species for the production of this antibody is Mouse. The antigen used for immunization is Bovine Bovine HSP70. The antibody is tested and validated for WB, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:100). This MAb for HSP70 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP25/HSP27 |
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| SMC-114D-A488 | Stressmarq | 0.1mg | EUR 426 |
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Description: A monoclonal antibody from clone 8A7 against Human | Mouse | Rat HSP25/HSP27. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HSP27 peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, FCM, FACS assays with the following recommended dilutions: WB (1:5000), ICC/IF (1:200), IHC (1:100). This MAb for HSP25/HSP27 is conjugated with ATTO 488. |
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Monoclonal antibody for CD74 |
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| SMC-116D-A488 | Stressmarq | 0.1mg | EUR 410.4 |
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Description: A monoclonal antibody from clone PIN.1 against Human | Mouse | Rat | Bovine | Dog | Hamster | Monkey | Guinea Pig (Cavia porcellus) | Sheep | Pig | African clawed frog (Xenopus laevis) | Mollusk | Mussel (Perna viridis) CD74. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human CD74 invariant chain synthetic peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, FCM, FACS assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:50). This MAb for CD74 is conjugated with ATTO 488. |
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Monoclonal antibody for HSF1 |
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| SMC-118D-A488 | Stressmarq | 0.1mg | EUR 398.4 |
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Description: A monoclonal antibody from clone 10H8 against Human | Rat | Mouse | Bovine | Fungi HSF1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, GS assays with the following recommended dilutions: WB (1:1000), IHC (1:1000), ICC/IF (1:200). This MAb for HSF1 is conjugated with ATTO 488. |
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Monoclonal antibody for HSF2 |
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| SMC-119D-A488 | Stressmarq | 0.1mg | EUR 398.4 |
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Description: A monoclonal antibody from clone 3E2 against Human | Rat | Mouse | Bovine | Monkey | Invertebrate | Coral (Anthozoa) | Dog | Hamster | Pig | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Mollusk | Mussel (Perna viridis) | Fish (O. melastigma) HSF2. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified recombinant mouse HSF2 protein. The antibody is tested and validated for WB, ICC/IF, AM assays with the following recommended dilutions: WB (1:250), ICC/IF (1:200). This MAb for HSF2 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP70/HSC70 (Plant) |
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| SMC-120D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 5G1-95 against Human | Rat | Mouse | Bovine | Dog | Chicken | Fruit Fly (Drosophila melanogaster) | Guinea Pig (Cavia porcellus) | Pig | Hamster | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Squirrel | Invertebrate | Sea squirt (Ciona intestinalis) HSP70/HSC70 (Plant). The host species for the production of this antibody is Mouse. The antigen used for immunization is Plant Purified HSP70 from Phaseolus aureus (mung bean). The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000). This MAb for HSP70/HSC70 (Plant) is conjugated with ATTO 488. |
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Monoclonal antibody for PSD95 |
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| SMC-122D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 6G6 against Human | Rat | Mouse | Bovine | Dog | Chicken | Gerbil | Guinea Pig (Cavia porcellus) | Pig | Hamster | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Frog | Wood Frog (Rana sylvatica) PSD95. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Recombinant rat PSD-95. The antibody is tested and validated for WB, IHC, ICC/IF, AM assays with the following recommended dilutions: WB (1:250), IHC (1:1000), ICC/IF (1:100). This MAb for PSD95 is conjugated with ATTO 488. |
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Monoclonal antibody for PSD95 |
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| SMC-123D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 7E3 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Rabbit | Guinea Pig (Cavia porcellus) | Pig | Dog | Sheep | Chicken | African clawed frog (Xenopus laevis) | Yeast | Fruit Fly (Drosophila melanogaster) | Fish | Rainbow Trout (Oncorhynchus mykiss) PSD95. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Recombinant rat PSD-95. The antibody is tested and validated for WB, IHC, ICC/IF, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:1000), ICC/IF (1:100). This MAb for PSD95 is conjugated with ATTO 488. |
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Monoclonal antibody for CaMKII (Alpha-Specific) |
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| SMC-124D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 6G9 against Human | Mouse | Rat | Bovine | Sheep | Chicken | Fruit Fly (Drosophila melanogaster) | African clawed frog (Xenopus laevis) CaMKII (Alpha-Specific). The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Partially purified rat CaMKII. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, RIA assays with the following recommended dilutions: WB (1:10000), IHC (1:2000), ICC/IF (1:50). This MAb for CaMKII (Alpha-Specific) is conjugated with ATTO 488. |
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Monoclonal antibody for CaMKII |
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| SMC-125D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 22B1 against Human | Mouse | Rat | Bovine | Dog | Chicken | Guinea Pig (Cavia porcellus) | Monkey | Pig | Hamster | Rabbit | Sheep CaMKII. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Synthetic peptide. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000). This MAb for CaMKII is conjugated with ATTO 488. |
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Monoclonal antibody for KDEL Receptor |
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| SMC-129D-A488 | Stressmarq | 0.1mg | EUR 462 |
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Description: A monoclonal antibody from clone KR-10 against Human | Mouse | Rat KDEL Receptor. The host species for the production of this antibody is Mouse. The antigen used for immunization is Bovine A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH. The antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:1000). This MAb for KDEL Receptor is conjugated with ATTO 488. |
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Monoclonal antibody for HO-1 |
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| SMC-131D-A488 | Stressmarq | 0.1mg | EUR 483.6 |
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Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 488. |
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Monoclonal antibody for Grp75 |
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| SMC-133D-A488 | Stressmarq | 0.1mg | EUR 474 |
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Description: A monoclonal antibody from clone S52A-42 against Human | Mouse | Rat | Bovine | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Pig | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) Grp75. The host species for the production of this antibody is Mouse. The antigen used for immunization is Mouse Fusion protein amino acids 551-766 of mouse SALM2.. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:200). This MAb for Grp75 is conjugated with ATTO 488. |
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Monoclonal antibody for SAP102 |
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| SMC-134D-A488 | Stressmarq | 0.1mg | EUR 406.8 |
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Description: A monoclonal antibody from clone S19-2 against Human | Mouse | Rat SAP102. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Fusion protein amino acids 1-120 of rat SAP102. The antibody is tested and validated for WB, IHC, IP, ICC/IF, AM assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for SAP102 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 alpha/beta |
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| SMC-135D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
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Description: A monoclonal antibody from clone Hyb-K41220A against Human HSP90 alpha/beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304. The antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HSP90 alpha/beta is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 beta |
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| SMC-136D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
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Description: A monoclonal antibody from clone Hyb-K3701 against Human | Mouse HSP90 beta. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human HSP90beta; Specificity mapped to amino acids 185-335. The antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:3000). This MAb for HSP90 beta is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 |
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| SMC-137D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
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Description: A monoclonal antibody from clone D7A against Human | Mouse | Rat | Bovine HSP90. The host species for the production of this antibody is Mouse. The antigen used for immunization is Chicken Full length protein HSP90 purified from chicken brain. The antibody is tested and validated for WB, IHC, IP, ELISA, AM assays with the following recommended dilutions: WB (1:500), IP (5µg) . This MAb for HSP90 is conjugated with ATTO 488. |
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Monoclonal antibody for FKBP51 |
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| SMC-138D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
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Description: A monoclonal antibody from clone Hi51B against Human | Mouse | Rat | Bovine FKBP51. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Synthetic peptide corresponding to the residues of human FKBP51. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:1000). This MAb for FKBP51 is conjugated with ATTO 488. |
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Monoclonal antibody for FKBP52 |
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| SMC-139D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
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Description: A monoclonal antibody from clone Hi52C against Human | Mouse | Rat | Bovine | Chicken | Dog | Guinea Pig (Cavia porcellus) | Hamster | Monkey | Pig | Rabbit | Sheep | African clawed frog (Xenopus laevis) FKBP52. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Synthetic peptide corresponding to the residues of human FKBP52. The antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg). This MAb for FKBP52 is conjugated with ATTO 488. |
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Monoclonal antibody for LAMP1 |
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| SMC-140D-A488 | Stressmarq | 0.1mg | EUR 468 |
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Description: A monoclonal antibody from clone Ly1C6 against Species Independent LAMP1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Rat Rat liver lysosomal membrane preparations. The antibody is tested and validated for WB, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:1000). This MAb for LAMP1 is conjugated with ATTO 488. |
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Monoclonal antibody for LAMP2 |
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| SMC-141D-A488 | Stressmarq | 0.1mg | EUR 454.8 |
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Description: A monoclonal antibody from clone GL2A7 against Species Independent LAMP2. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Purified preparation of mouse liver lysosomal membranes. The antibody is tested and validated for WB, ICC/IF, IP assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:500). This MAb for LAMP2 is conjugated with ATTO 488. |
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Monoclonal antibody for Alpha A Crystallin |
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| SMC-142B-A488 | Stressmarq | 0.2mg | EUR 489.6 |
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Description: A monoclonal antibody from clone 1H3.B8 against Species Independent Alpha A Crystallin. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Native Alpha Crystallin. The antibody is tested and validated for WB, ELISA, ICC/IF assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for Alpha A Crystallin is conjugated with ATTO 488. |
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Monoclonal antibody for HSP40, Hdj1 |
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| SMC-145D-A488 | Stressmarq | 0.1mg | EUR 441.6 |
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Description: A monoclonal antibody from clone 3B9.E6 against Species Independent HSP40, Hdj1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant Protein HSP40 (Hdj1). The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for HSP40, Hdj1 is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 alpha |
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| SMC-147B-A488 | Stressmarq | 0.2mg | EUR 543.6 |
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Description: A monoclonal antibody from clone 2G5.G3 against Species Independent HSP90 alpha. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HSP90alpha. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This MAb for HSP90 alpha is conjugated with ATTO 488. |
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Monoclonal antibody for HSP90 (total) |
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| SMC-149B-A488 | Stressmarq | 0.2mg | EUR 543.6 |
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Description: A monoclonal antibody from clone 4F3.E8 against Human | Mouse | Rat HSP90 (total). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant Human HSP90 purified from E.coli. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:2000), IHC (1:100), ICC/IF (1:100). This MAb for HSP90 (total) is conjugated with ATTO 488. |
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Monoclonal antibody for HSP40, YDJ1 |
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| SMC-150D-A488 | Stressmarq | 0.1mg | EUR 471.6 |
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Description: A monoclonal antibody from clone 1G10.H8 against Human | Rat | Rainbow Trout (Oncorhynchus mykiss) HSP40, YDJ1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Yeast Full length protein yeast HSP40 (YDJ1). The antibody is tested and validated for WB, IP, ELISA assays with the following recommended dilutions: WB (1:2000). This MAb for HSP40, YDJ1 is conjugated with ATTO 488. |
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Monoclonal antibody for HSC70 (HSP73) |
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| SMC-151B-A488 | Stressmarq | 0.2mg | EUR 561.6 |
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Description: A monoclonal antibody from clone 1F2-H5 against Human | Mouse | Chicken HSC70 (HSP73). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length human HSC70. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, PLA, PBA, AM assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for HSC70 (HSP73) is conjugated with ATTO 488. |
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Monoclonal antibody for p38 alpha (MAP Kinase) |
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| SMC-152D-A488 | Stressmarq | 0.1mg | EUR 411.6 |
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Description: A monoclonal antibody from clone 9F12 against Human | Mouse | Rat p38 alpha (MAP Kinase). The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Full length recombinant protein expressed in E.coli cells. The antibody is tested and validated for WB, IHC, IP, ELISA, AM assays with the following recommended dilutions: WB (1:1000), IHC (1:1000). This MAb for p38 alpha (MAP Kinase) is conjugated with ATTO 488. |
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Monoclonal antibody for Acetylated Lysine |
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| SMC-153D-A488 | Stressmarq | 0.1mg | EUR 478.8 |
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Description: A monoclonal antibody from clone 7F8 against Human | Mouse | Rat | Bovine | Monkey Acetylated Lysine. The host species for the production of this antibody is Mouse. The antigen used for immunization is Acetylated KLH. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100). This MAb for Acetylated Lysine is conjugated with ATTO 488. |
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Monoclonal antibody for Nitrotyrosine |
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| SMC-154D-A488 | Stressmarq | 0.1mg | EUR 492 |
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Description: A monoclonal antibody from clone 39B6 against Human | Mouse | Rat | Bovine Nitrotyrosine. The host species for the production of this antibody is Mouse. The antigen used for immunization is 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, FCM, AM assays with the following recommended dilutions: WB (1:1400), IHC (1:100). This MAb for Nitrotyrosine is conjugated with ATTO 488. |
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Monoclonal antibody for DNA/RNA Damage |
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| SMC-155D-A488 | Stressmarq | 0.1mg | EUR 453.6 |
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488. |
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Monoclonal antibody for p23 |
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| SMC-156D-A488 | Stressmarq | 0.1mg | EUR 417.6 |
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Description: A monoclonal antibody from clone JJ6 against Human | Mouse | Rat | Bovine p23. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human Recombinant human full length p23 protein. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA, AM assays with the following recommended dilutions: WB (1:2000) IHC (1:100), ICC/IF (1:100). This MAb for p23 is conjugated with ATTO 488. |
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The common last accuracy achieved for the coaching set was 97%, and that for the testing set was 92%. As well as, 5 exterior knowledge units have been used to additional validate the fashions. In the end, 14 consultant structural options (or guidelines) have been decided to effectively predict autofluorescence in knowledge units containing each fluorescent and nonfluorescent compounds. A number of circumstances have been illustrated on this examine to show the applicability of those guidelines.
